pJBpn25lacZ vector (V005363)

LOCUS       40924_25941       12509 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJBpn25lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12509)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12509)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 12509)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(796..1983)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2031..2059)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(2072..2088)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2699..3844)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(3953..4002)
                     /label=neo
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(4236..4766)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     6010..6026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6090..7169)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(7170..7247)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        7305..7349
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    7357..7373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7424..7446
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7463..10507
                     /label=lacZ
                     /note="beta-galactosidase"
     promoter        complement(10946..10963)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      complement(10948..10965)
                     /label=T3
                     /note="T3"
                     /regulatory_class="promoter"
     terminator      10982..11029
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      11103..11150
                     /label=T7T
                     /note="T7T"
                     /regulatory_class="terminator"
     terminator      11103..11150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      11222..11270
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      11329..11410
                     /label=fdT
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      11332..11380
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(11943..12047)
                     /label=AmpR promoter
     oriT            12224..12332
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(12419..12435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"