LOCUS       40924_25941       12509 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJBpn25lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12509)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12509)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 12509)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(796..1983)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2031..2059)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(2072..2088)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2699..3844)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(3953..4002)
                     /label=neo
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(4236..4766)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     6010..6026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6090..7169)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(7170..7247)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        7305..7349
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    7357..7373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7424..7446
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7463..10507
                     /label=lacZ
                     /note="beta-galactosidase"
     promoter        complement(10946..10963)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      complement(10948..10965)
                     /label=T3
                     /note="T3"
                     /regulatory_class="promoter"
     terminator      10982..11029
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      11103..11150
                     /label=T7T
                     /note="T7T"
                     /regulatory_class="terminator"
     terminator      11103..11150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      11222..11270
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      11329..11410
                     /label=fdT
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      11332..11380
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(11943..12047)
                     /label=AmpR promoter
     oriT            12224..12332
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(12419..12435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
       61 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
      121 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
      181 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
      241 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
      301 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
      361 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
      421 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
      481 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
      541 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
      601 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc
      661 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
      721 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
      781 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
      841 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga
      901 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc
      961 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     1021 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     1081 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc
     1141 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     1201 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     1261 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     1321 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     1381 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg
     1441 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     1501 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     1561 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     1621 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     1681 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
     1741 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
     1801 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
     1861 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
     1921 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
     1981 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
     2041 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact
     2101 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2161 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2221 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2281 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc
     2701 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     2761 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     2821 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     2881 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     2941 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     3001 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     3061 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     3121 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     3181 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     3241 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     3301 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     3361 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     3421 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     3481 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     3541 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     3601 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     3661 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
     3721 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     3781 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     3841 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct
     3901 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     3961 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     4021 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     4081 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4141 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga
     4201 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg
     4261 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca
     4321 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     4381 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     4441 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     4501 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     4561 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     4621 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     4681 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
     4741 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct
     4801 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtctaa gaaaccatta
     5581 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     5641 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     5701 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     5761 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     5821 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     5881 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     5941 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6001 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gacaattcgc
     6061 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6121 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
     6181 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
     6241 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
     6301 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
     6361 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
     6421 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
     6481 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
     6541 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
     6601 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
     6661 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
     6721 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
     6781 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
     6841 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
     6901 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
     6961 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
     7021 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
     7081 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
     7141 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
     7201 cgctatcatg ccataccgcg aaaggttttg caccattcga tggtgtcaac gtaaatgcat
     7261 gccgcttcgc cttcgcgcgc gaattgtcga ctctagtcga gaaatcataa aaaatttatt
     7321 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
     7381 cagaattata gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga
     7441 aggagatata catatggatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7501 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7561 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
     7621 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
     7681 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
     7741 caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
     7801 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
     7861 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
     7921 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
     7981 aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga
     8041 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
     8101 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
     8161 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
     8221 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
     8281 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     8341 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     8401 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     8461 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     8521 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     8581 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     8641 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     8701 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     8761 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     8821 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     8881 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     8941 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     9001 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     9061 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     9121 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     9181 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     9241 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     9301 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     9361 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     9421 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     9481 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     9541 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     9601 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     9661 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     9721 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     9781 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     9841 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     9901 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     9961 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
    10021 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
    10081 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
    10141 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
    10201 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
    10261 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
    10321 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
    10381 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
    10441 gtcagtatcg gcgnnnnnnc agctgagcgc cggtcgctac cattaccagt tggtctggtg
    10501 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
    10561 tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt
    10621 ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat
    10681 cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca
    10741 accgctgttt ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat
    10801 taccggcatg ttagtgatgt ttgcgccgtt ctttattttt atcttcgggc cactgttaca
    10861 atacaacatt ttagtaggat cgattgttgg tggtatttat ctaggctttt gttttaacgc
    10921 cggggccgcg gccgacttgg ccttcccttt agtgagggtt aataaacttg gtgagcaata
    10981 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
    11041 aactatatgc gctcatacga tatgaacgtt gagactgccg ctgagttatc agtgagcaat
    11101 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
    11161 gaactatatc cggatagctt atcgctagag gtcgaaattc acctcgaaag caagctgata
    11221 aaccgataca attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa
    11281 aaattattat tcgcaattcc aagctaattc acctcgaaag caagctgata aaccgataca
    11341 attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat
    11401 tcgcaattcc aagctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11461 gcagctcccg gagacggtca cagcttgtct gtaagcggat gcagatcacg cgccctgtag
    11521 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    11581 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccactca
    11641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    11701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    12001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    12061 aaagtgccac ctgggtcgat cgacggatct tttccgctgc ataaccctgc ttcggggtca
    12121 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
    12181 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
    12241 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
    12301 tcaacgggaa tcctgctctg cgaggctggc cgtaagctct aagaaaccat tattatcatg
    12361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attaattcac
    12421 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    12481 ttgcagcaca tccccctttc gccagcaga
//
