pJB3kan2 vector (V005370)

LOCUS       40924_25901        6070 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJB3kan2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    236..257
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        272..302
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     334..350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(363..419)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             562..1374
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(1702..1718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2329..3474)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(3583..3632)
                     /label=Tn5 neomycin promoter
                     /note="Tn5 neomycin promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(3866..4396)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(4646..5503)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5504..5608)
                     /label=AmpR promoter
     oriT            5785..5893
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(5980..5996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"