LOCUS       40924_25901        6070 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJB3kan2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    236..257
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        272..302
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     334..350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(363..419)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             562..1374
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(1702..1718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2329..3474)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(3583..3632)
                     /label=Tn5 neomycin promoter
                     /note="Tn5 neomycin promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(3866..4396)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(4646..5503)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5504..5608)
                     /label=AmpR promoter
     oriT            5785..5893
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(5980..5996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
       61 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      361 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcc
      421 ccggatccgt cgacctgcag gggggggggg gaaagccacg ttgtgtctca aaatctctga
      481 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      541 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg
      601 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      661 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
      721 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
      781 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      841 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
      901 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      961 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1021 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1081 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
     1141 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1201 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1261 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1321 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1381 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcggc
     1441 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     1501 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     1561 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct
     1621 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgccccccc ccccctgcag
     1681 gtcgacggat ccggggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1741 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     1861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc
     1921 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     1981 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2041 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     2101 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     2161 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2281 tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
     2341 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
     2401 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
     2461 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
     2521 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
     2581 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
     2641 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
     2701 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
     2761 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
     2821 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
     2881 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
     2941 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
     3001 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
     3061 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     3121 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     3181 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag
     3241 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     3301 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     3361 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     3421 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     3481 tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct tgatcccctg
     3541 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     3601 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     3661 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     3721 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     3781 ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga ggggcgcagc
     3841 ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
     3901 cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca aggtttataa
     3961 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     4021 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     4081 ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     4141 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     4201 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     4261 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     4321 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgtggtatc
     4381 cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg
     4441 tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     5641 tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt
     5701 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc
     5761 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
     5821 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
     5881 gcgaggctgg ccgtaagctc taagaaacca ttattatcat gacattaacc tataaaaata
     5941 ggcgtatcac gaggcccttt cgtcttcaag aattaattca ctggccgtcg ttttacaacg
     6001 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6061 cgccagcaga
//
