Basic Vector Information
- Vector Name:
- PJAC93
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7589 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
- Promoter:
- T3
PJAC93 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PJAC93 vector Sequence
LOCUS 40924_25846 7589 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector PJAC93, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7589) AUTHORS Moser F, Horwitz A, Chen J, Lim W, Voigt CA. TITLE Genetic sensor for strong methylating compounds JOURNAL ACS Synth Biol 2 (10), 614-624 (2013) PUBMED 24032656 REFERENCE 2 (bases 1 to 7589) AUTHORS Moser F, Horwitz A, Chen J, Lim WA, Voigt CA. TITLE Direct Submission JOURNAL Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 7589) TITLE Direct Submission REFERENCE 4 (bases 1 to 7589) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D, Cambridge, MA 02139, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7589 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 434..1934 /label=CG TRP1 w promoter and terminator /note="CG TRP1 w promoter and terminator" misc_feature 1941..2027 /label=3x Ada operator /note="3x Ada operator" regulatory 2028..2274 /label=PCyc1 promoter /note="PCyc1 promoter" /regulatory_class="promoter" CDS 2293..3000 /label=GFP /note="Aequorea victoria green fluorescent protein" regulatory 3045..3739 /label=C. albicans TadH1 terminator /note="C. albicans TadH1 terminator" /regulatory_class="terminator" promoter complement(4137..4155) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4162..4178) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4319..4774 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5412..5516 /label=AmpR promoter CDS 5517..6374 /label=AmpR /note="beta-lactamase" rep_origin 6548..7136 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7424..7445 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 7460..7490 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7498..7514 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7522..7538 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 7559..7577 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase"
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