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PJAC93 vector (V005382#)

Basic Vector Information

Basic Vector Information
Vector Name PJAC93 Antibiotic Resistance Ampicillin
Length 7589 bp Type Cloning vector
Source Moser F, Horwitz A, Chen J, Lim W, Voigt CA.

PJAC93 vector Vector Map

CG TRP1 w promoter and terminator 3x Ada operator PCyc1 promoter EGFP C. albicans TadH1 terminator T7 promoter M13 fwd f1 ori AmpR promoter AmpR ColE1 origin of replication CAP binding site lac promoter lac operator M13 rev T3 promoter

PJAC93 vector Sequence

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LOCUS       Exported                7589 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PJAC93, complete sequence.
ACCESSION   KF322089
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7589)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Cloning vector PJAC93"
                     /mol_type="other DNA"
                     /db_xref="taxon:1403154"
     misc_feature    434..1934
                     /label=CG TRP1 w promoter and terminator
                     /note="CG TRP1 w promoter and terminator"
     misc_feature    1941..2027
                     /label=3x Ada operator
                     /note="3x Ada operator"
     regulatory      2028..2274
                     /regulatory_class="promoter"
                     /note="PCyc1 promoter"
     CDS             2275..3012
                     /codon_start=1
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AGW27654.1"
                     /translation="MTELETSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
                     NGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYNGS"
     CDS             2293..3000
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
     regulatory      3045..3739
                     /regulatory_class="terminator"
                     /note="C. albicans TadH1 terminator"
     promoter        complement(4137..4155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4162..4178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4319..4774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4324..4764
                     /label=F1 ori
                     /note="F1 ori"
     promoter        5412..5516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5517..6377
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5715..6377
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="AGW27655.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      6472..7154
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      6548..7136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7424..7445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7460..7490
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7498..7514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7522..7538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7559..7577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"

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