LOCUS       40924_25846        7589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PJAC93, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    434..1934
                     /label=CG TRP1 w promoter and terminator
                     /note="CG TRP1 w promoter and terminator"
     misc_feature    1941..2027
                     /label=3x Ada operator
                     /note="3x Ada operator"
     regulatory      2028..2274
                     /label=PCyc1 promoter
                     /note="PCyc1 promoter"
                     /regulatory_class="promoter"
     CDS             2293..3000
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      3045..3739
                     /label=C. albicans TadH1 terminator
                     /note="C. albicans TadH1 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4137..4155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4162..4178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4319..4774
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5412..5516
                     /label=AmpR promoter
     CDS             5517..6374
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6548..7136
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7424..7445
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7460..7490
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7498..7514
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7522..7538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7559..7577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggccgcatc ttgcgcttta atttttcggg ccgcatcttg
     1981 cgctttaatt tttcgggccg catcttgcgc tttaattttt cgggcccgag cagatccgcc
     2041 aggcgtgtat atatagcgtg gatggccagg caactttagt gctgacacat acaggcatat
     2101 atatatgtgt gcgacgacac atgatcatat ggcatgcatg tgctctgtat gtatataaaa
     2161 ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggacc tttgcagcat
     2221 aaattactat acttctatag acacacaaac acaaatacac acactaaatt aataatgact
     2281 gaactcgaga ctagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     2341 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     2401 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     2461 cctacacttg tcactacttt gtgttatggt gttcaatgct tttcaagata cccagatcac
     2521 atgaaaaggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     2581 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     2641 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     2701 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     2761 aagaatggaa tcaaagttaa tttcaaaact agacacaaca ttgaagatgg aagcgttcaa
     2821 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     2881 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     2941 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     3001 aatggatcct gagtaaataa gcggccgcca ccgcggtgga gctctaagca aatagctaaa
     3061 ttatatacga attaatatta tgattaagtg tttacgtgag tgcgatattt ttattactat
     3121 cttatacagt tgtatatact ctataaaatg agttgtctat taattaacgc gatgaatgct
     3181 ttctgggttt acctctccaa caactctagt ttacttctca atacattcaa ttgtatttga
     3241 tttgtcaata cttcatcatt aatcaattct atagttttgt ttttctcgtt tatttccaaa
     3301 tttaatgcat caattttatt attcaatttg tcgttgattt tggttaatga ttttatggtt
     3361 tgatctctgg cattgattgt ttgtgttagt ttttcattat tgataattaa attatttaag
     3421 ttagttatca actcggtgtt ttcaagtttc aagttttcaa tttctttaga gtttattaga
     3481 tttgtcaaag tttctgaatt gcttgattgg tcctgtagaa gagtatttgt tgttgtggat
     3541 aattgattca atttttgaga caattgctgg aaggcgttga aatatctagc atcaatctca
     3601 tggttttttt cccgagagtc tcgtagattc aattgtttta atatatcttg ggaccactct
     3661 tgatttgaac tcatggaaat taaactgggt gttgtgttgt ggtgtaatga ttgtaccccc
     3721 tttgcttata attgtgtggt tccgatgctg acttactggg tattatatgt gtgcccaata
     3781 gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata
     3841 aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg
     3901 atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt
     3961 cgtggcgaga ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa
     4021 aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt
     4081 ttgattcaga aggccggcca tttaaatgtt taaacggcca cgctggccca attcgcccta
     4141 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4201 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4261 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     4321 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4381 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4441 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4501 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4561 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4621 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4681 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4741 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     4801 atctgtgcgg tatttcacac cgcatagggt aataactgat ataattaaat tgaagctcta
     4861 atttgtgagt ttagtattag gatctctacc cttggcgaaa agtcctctgc caacaatgat
     4921 gatatctgat ccaccactta caacttcgtc gacggttctg tactgctgac ccaatatgcg
     4981 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     5041 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     5101 attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5161 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5221 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5281 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5341 gtcatgataa taatggtttc ttagacgtcg gcgcgccgag gacgtcaggt ggcacttttc
     5401 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5461 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5521 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5581 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5641 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5701 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5761 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5821 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5881 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5941 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6001 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6061 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6121 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6181 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6241 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6301 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6361 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6421 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6661 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7141 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7201 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7261 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7321 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7381 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7441 ctcattaggc accccaggct ttacacttta tgcttccggc tcctatgttg tgtggaattg
     7501 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcggaa
     7561 ttaaccctca ctaaagggaa caaaagctg
//
