PJAC92 vector (V005383)

Basic Vector Information

      • Vector Name:
      • PJAC92
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7535 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Moser F, Horwitz A, Chen J, Lim W, Voigt CA.

PJAC92 vector Vector Map

PJAC927535 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500CG TRP1 w promoter and terminator1x Ada operatorPcyc1 promoterEGFPC. albicans TadH1 terminatorT7 promoterM13 fwdf1 oriAmpR promoterAmpRColE1 origin of replicationCAP binding sitelac promoterlac operatorM13 revT3 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PJAC92 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7535 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PJAC92, complete sequence.
ACCESSION   KF322088
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Cloning vector PJAC92"
                     /mol_type="other DNA"
                     /db_xref="taxon:1403153"
     misc_feature    434..1934
                     /label=CG TRP1 w promoter and terminator
                     /note="CG TRP1 w promoter and terminator"
     misc_feature    1941..1973
                     /label=1x Ada operator
                     /note="1x Ada operator"
     regulatory      1974..2220
                     /regulatory_class="promoter"
                     /note="Pcyc1 promoter"
     CDS             2221..2958
                     /codon_start=1
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AGW27652.1"
                     /translation="MTELETSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
                     NGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYNGS"
     CDS             2239..2946
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
     regulatory      2991..3685
                     /regulatory_class="terminator"
                     /note="C. albicans TadH1 terminator"
     promoter        complement(4083..4101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4108..4124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4265..4720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4270..4710
                     /label=F1 ori
                     /note="F1 ori"
     promoter        5358..5462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5463..6323
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5661..6323
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="AGW27653.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      6418..7100
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      6494..7082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7370..7391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7406..7436
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7444..7460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7468..7484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7505..7523
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"

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