Basic Vector Information
- Vector Name:
- PJAC92
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7535 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
PJAC92 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PJAC92 vector Sequence
LOCUS 40924_25841 7535 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector PJAC92, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7535)
AUTHORS Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
TITLE Genetic sensor for strong methylating compounds
JOURNAL ACS Synth Biol 2 (10), 614-624 (2013)
PUBMED 24032656
REFERENCE 2 (bases 1 to 7535)
AUTHORS Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
TITLE Direct Submission
JOURNAL Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech
Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 7535)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7535)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
Cambridge, MA 02139, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7535
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 434..1934
/label=CG TRP1 w promoter and terminator
/note="CG TRP1 w promoter and terminator"
misc_feature 1941..1973
/label=1x Ada operator
/note="1x Ada operator"
regulatory 1974..2220
/label=Pcyc1 promoter
/note="Pcyc1 promoter"
/regulatory_class="promoter"
CDS 2239..2946
/label=GFP
/note="Aequorea victoria green fluorescent protein"
regulatory 2991..3685
/label=C. albicans TadH1 terminator
/note="C. albicans TadH1 terminator"
/regulatory_class="terminator"
promoter complement(4083..4101)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4108..4124)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4265..4720
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5358..5462
/label=AmpR promoter
CDS 5463..6320
/label=AmpR
/note="beta-lactamase"
rep_origin 6494..7082
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7370..7391
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7406..7436
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7444..7460
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7468..7484
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 7505..7523
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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