LOCUS       40924_25841        7535 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PJAC92, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    434..1934
                     /label=CG TRP1 w promoter and terminator
                     /note="CG TRP1 w promoter and terminator"
     misc_feature    1941..1973
                     /label=1x Ada operator
                     /note="1x Ada operator"
     regulatory      1974..2220
                     /label=Pcyc1 promoter
                     /note="Pcyc1 promoter"
                     /regulatory_class="promoter"
     CDS             2239..2946
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      2991..3685
                     /label=C. albicans TadH1 terminator
                     /note="C. albicans TadH1 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4083..4101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4108..4124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4265..4720
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5358..5462
                     /label=AmpR promoter
     CDS             5463..6320
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6494..7082
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7370..7391
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7406..7436
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7444..7460
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7468..7484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7505..7523
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggccgcatc ttgcgcttta atttttcggg cccgagcaga
     1981 tccgccaggc gtgtatatat agcgtggatg gccaggcaac tttagtgctg acacatacag
     2041 gcatatatat atgtgtgcga cgacacatga tcatatggca tgcatgtgct ctgtatgtat
     2101 ataaaactct tgttttcttc ttttctctaa atattctttc cttatacatt aggacctttg
     2161 cagcataaat tactatactt ctatagacac acaaacacaa atacacacac taaattaata
     2221 atgactgaac tcgagactag taaaggagaa gaacttttca ctggagttgt cccaattctt
     2281 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     2341 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     2401 ccatggccta cacttgtcac tactttgtgt tatggtgttc aatgcttttc aagataccca
     2461 gatcacatga aaaggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     2521 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     2581 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     2641 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     2701 aaacaaaaga atggaatcaa agttaatttc aaaactagac acaacattga agatggaagc
     2761 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2821 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2881 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2941 ctatacaatg gatcctgagt aaataagcgg ccgccaccgc ggtggagctc taagcaaata
     3001 gctaaattat atacgaatta atattatgat taagtgttta cgtgagtgcg atatttttat
     3061 tactatctta tacagttgta tatactctat aaaatgagtt gtctattaat taacgcgatg
     3121 aatgctttct gggtttacct ctccaacaac tctagtttac ttctcaatac attcaattgt
     3181 atttgatttg tcaatacttc atcattaatc aattctatag ttttgttttt ctcgtttatt
     3241 tccaaattta atgcatcaat tttattattc aatttgtcgt tgattttggt taatgatttt
     3301 atggtttgat ctctggcatt gattgtttgt gttagttttt cattattgat aattaaatta
     3361 tttaagttag ttatcaactc ggtgttttca agtttcaagt tttcaatttc tttagagttt
     3421 attagatttg tcaaagtttc tgaattgctt gattggtcct gtagaagagt atttgttgtt
     3481 gtggataatt gattcaattt ttgagacaat tgctggaagg cgttgaaata tctagcatca
     3541 atctcatggt ttttttcccg agagtctcgt agattcaatt gttttaatat atcttgggac
     3601 cactcttgat ttgaactcat ggaaattaaa ctgggtgttg tgttgtggtg taatgattgt
     3661 accccctttg cttataattg tgtggttccg atgctgactt actgggtatt atatgtgtgc
     3721 ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
     3781 catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
     3841 aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
     3901 atgagtcgtg gcgagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
     3961 ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
     4021 ccttgtttga ttcagaaggc cggccattta aatgtttaaa cggccacgct ggcccaattc
     4081 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     4141 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4201 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4261 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4681 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     4741 ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa ttaaattgaa
     4801 gctctaattt gtgagtttag tattaggatc tctacccttg gcgaaaagtc ctctgccaac
     4861 aatgatgata tctgatccac cacttacaac ttcgtcgacg gttctgtact gctgacccaa
     4921 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     4981 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     5041 tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5101 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5161 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5221 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5281 gttaatgtca tgataataat ggtttcttag acgtcggcgc gccgaggacg tcaggtggca
     5341 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5521 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5581 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5641 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5701 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5761 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5821 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5881 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5941 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6001 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6061 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6121 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6181 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6241 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6301 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7201 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7261 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7321 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7381 agctcactca ttaggcaccc caggctttac actttatgct tccggctcct atgttgtgtg
     7441 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7501 tcggaattaa ccctcactaa agggaacaaa agctg
//
