pIS421 vector (V005390)

LOCUS       40924_25786        6187 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast selection vector pIS421, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Sadowski I, Lourenco P, Parent J.
  TITLE     Dominant marker vectors for selecting yeast mating products
  JOURNAL   Yeast 25 (8), 595-599 (2008)
  PUBMED    18613257
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Sadowski I, Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2008) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 6187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "8"; pages: "595-599"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-2008) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..845
                     /label=ARS1/trp1
                     /note="ARS1/trp1"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1245..1349
                     /label=AmpR promoter
     CDS             1350..2207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2381..2969
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3227..3245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3507..3704)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3758..4210)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVA
                     EVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKS
                     VVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDF"
     promoter        complement(4265..4608)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             complement(5165..5965)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5966..6182)
                     /label=URA3 promoter
     rep_origin      6184..6187
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"