LOCUS       40924_25786        6187 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast selection vector pIS421, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Sadowski I, Lourenco P, Parent J.
  TITLE     Dominant marker vectors for selecting yeast mating products
  JOURNAL   Yeast 25 (8), 595-599 (2008)
  PUBMED    18613257
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Sadowski I, Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2008) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 6187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "8"; pages: "595-599"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-2008) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..845
                     /label=ARS1/trp1
                     /note="ARS1/trp1"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1245..1349
                     /label=AmpR promoter
     CDS             1350..2207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2381..2969
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3227..3245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3507..3704)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3758..4210)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVA
                     EVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKS
                     VVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDF"
     promoter        complement(4265..4608)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             complement(5165..5965)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5966..6182)
                     /label=URA3 promoter
     rep_origin      6184..6187
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
       61 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
      121 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
      181 ttaagtattg tttgtgcact tgcctgcagg ccttttgaaa agcaagcata aaagatctaa
      241 acataaaatc tgtaaaataa caagatgtaa agataatgct aaatcatttg gctttttgat
      301 tgattgtaca ggaaaatata catcgcaggg ggttgacttt taccatttca ccgcaatgga
      361 atcaaacttg ttgaagagaa tgttcacagg cgcatacgct acaatgaccc gattcttgct
      421 agccttttct cggtcttgca aacaaccgcc ggcagcttag tatataaata cacatgtaca
      481 tacctctctc cgtatcctcg taatcatttt cttgtattta tcgtcttttc gctgtaaaaa
      541 ctttatcaca cttatctcaa atacacttat taaccgcttt tactattatc ttctacgctg
      601 acagtaatat caaacagtga cacatattaa acacagtggt ttctttgcat aaacaccatc
      661 agcctcaagt cgtcaagtaa agatttcgtg ttcatgcaga tagataacaa tctatatgtt
      721 gataattagc gttgcctcat caatgcgaga tccgtttaac cggaccctag tgcacttacc
      781 ccacgttcgg tccactgtgt gccgaacatg ctccttcact attttaacat gtgggaattc
      841 ctcgacctgc agcgtacgaa gcttcagctg gcggccgcgt tctatagtgt cacctaaatc
      901 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
      961 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3181 cgcgttggcc gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact
     3241 atagggagac cggcagatcc gcggccgcat aggccactag tggatctgat atcatcgatg
     3301 aattcgagct cgagccgagc aggtcgcccg ttctgactcc cgagtccaac acctcctgca
     3361 ctgcctgctc gaccgcctcg ccggcagcaa ccatgttcaa cgacaacttc agcatcatgg
     3421 cagcagagag gatgcatccg atcgggttcg ccttcccggc gggcagatct cgacactgga
     3481 tggcggcgtt agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg
     3541 catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa
     3601 aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat
     3661 acaaatgaca agttcttgaa aacaagaatc tttttattgt cagtactgat tagatctggg
     3721 taactggcct aactggcctt ggaggagctg gcaactcaaa atccctttgc caaaaaccaa
     3781 catcatgcca tccaccatgc ttgtatccag ctgcgcgcaa tgtaccccgg gctgtgtatc
     3841 ccaaagcctc atgcaaccta acagatggat cgtttggaag gcctataaca gcaaccacag
     3901 acttaaaacc ttgcgcctcc atagacttaa gcaaatgtgt gtacaatgtg gatcctaggc
     3961 ccaacctttg atgcctatgt gacacgtaaa cagtactctc aactgtccaa tcgtaagcgt
     4021 tcctagcctt ccagggccca gcgtaagcaa taccagccac aacaccctca acctcagcaa
     4081 ccaaccaagg gtatctatct tgcaacctct ctagatcatc aatccactct tgtggtgttt
     4141 gtggctctgt cctaaagttc actgtagacg tctcaatgta atggttaacg atatcacaaa
     4201 ccgcggccat atcagctgct gtagctggcc taatctcaac tggtctcctc tccggagaac
     4261 ccatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg
     4321 aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg
     4381 cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtctg
     4441 cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa
     4501 tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga
     4561 gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgc tggccgggtg
     4621 acccggcggg gacgaggcaa gctaaacaga tctggcgcgc cttaattaac ccggggatcc
     4681 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
     4741 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
     4801 attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat
     4861 ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
     4921 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
     4981 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagctttttc tttccaattt
     5041 tttttttttc gtcattatag aaatcattac gaccgagatt cccgggtaat aactgatata
     5101 attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt
     5161 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
     5221 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
     5281 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
     5341 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     5401 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     5461 cagtaccctt agtatattct ccagtagcta gggagccctt gcatgacaat tctgctaaca
     5521 tcaaaaggcc tctaggttcc tttgttactt cttccgccgc ctgcttcaaa ccgctaacaa
     5581 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     5641 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     5701 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     5761 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     5821 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     5881 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     5941 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt
     6001 gcagttgggt taagaatact gggcaatttc atgtttcttc aacaccacat atgcgtatat
     6061 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgctcggag attaccgaat
     6121 caaaaaaatt tcaaagaaac cggaatcaaa aaaaagaaca aaaaaaaaaa agatgaattg
     6181 aaaagct
//
