pINTyrC vector (V005415)

LOCUS       40924_25591        4588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 4588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "761-766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2006) Biopathology, University of Palermo, via Divisi, 83, 
            Palermo 90100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..291
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     CDS             complement(443..1243)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1246..1455)
                     /label=URA3 promoter
     promoter        complement(1645..1975)
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     misc_feature    2005..2269
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        2682..2786
                     /label=AmpR promoter
     CDS             2787..3644
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3818..4406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"