LOCUS       40924_25591        4588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 4588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "761-766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2006) Biopathology, University of Palermo, via Divisi, 83, 
            Palermo 90100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..291
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     CDS             complement(443..1243)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1246..1455)
                     /label=URA3 promoter
     promoter        complement(1645..1975)
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     misc_feature    2005..2269
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        2682..2786
                     /label=AmpR promoter
     CDS             2787..3644
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3818..4406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagatcgtc
       61 gacatggaaa gtgatgttga agatgacgat aaagaaaccc aactaaagaa aagggctgta
      121 agcggtgtag aattgcgtat ttatgtttaa tatgtcatac ttttagcgct cattgttatt
      181 gtatactatt tatagctaac agtttattat tagatttcct ctaataagcg ttataccagt
      241 gtagtaagtt tatcacttca gctatgtgtc attttttttt tttttttttg aggatctaga
      301 gctttttctt tccaattttt tttttttcgt cattataaaa atcattacga ccgaaattcc
      361 cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
      421 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
      481 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
      541 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
      601 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
      661 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
      721 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
      781 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
      841 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
      901 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
      961 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     1021 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     1081 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     1141 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     1201 taggatgagt agcagcacgt tccttatatg tagctttcga catgattatt ttcgtttctg
     1261 caggtttttg ttctgtgcag ntgggttaga atactgagca atttcatgtt tcttcaacac
     1321 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgtctt ccttctgttc
     1381 ggagattacc gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa
     1441 aaatgatgaa ttgaaaagct ctagatcctc aaaaatcatc gcttcgctga ttaattaccc
     1501 cagaaataag gctaaaaaac taatcgcatt atcatcctat ggttgttaat ttgattcgtt
     1561 catttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc
     1621 tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag
     1681 gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac
     1741 ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag cgtattactg
     1801 aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac atttccacaa
     1861 catataagta agattagata tggatagtta tatggatatg tatatggtgg taatgccatg
     1921 taatatgatt attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgctcga
     1981 gaattccccg ggaagcttga tccttctttt gtgaaaagga gtatatttat ataactgaat
     2041 aaaaatatat attgtgacaa atgcccatgc aaaaaatcgt gaaaaaagga agaaagttct
     2101 actattctat gtaaaaataa acccattaga tttttttcct ctttgtgtaa tgctcactac
     2161 cgcgattatc gttcgtttca tctggacatg ccaacgttct tgacctttta gaacttacga
     2221 ccggatgaat actgtcatca tcacatgcaa gtaagttttt ggtcataaag tcgacgatct
     2281 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2341 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2461 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2521 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2581 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2641 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2701 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2761 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2821 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2881 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2941 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3001 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3061 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3121 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3181 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3241 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3301 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3361 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3421 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3481 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3541 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3601 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3661 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3781 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3841 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3901 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3961 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4021 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4081 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4141 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4201 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4261 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4321 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4381 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4441 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4501 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4561 gcgagtcagt gagcgaggaa gcggaaga
//
