pIgK3conaLUC vector (V005443)

LOCUS       40924_25441        6308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pIgK3conaLUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     gap             1635..1705
                     /estimated_length=71
     misc_feature    complement(1706..1750)
                     /label=3' UTR of LUC
                     /note="3' UTR of LUC"
     CDS             complement(1754..3403)
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      complement(3538..3701)
                     /label=CONA promoter
                     /note="CONA promoter"
                     /regulatory_class="promoter"
     regulatory      complement(3705..3757)
                     /label=IgK3 enhancer
                     /note="IgK3 enhancer"
                     /regulatory_class="promoter"
     primer_bind     complement(4079..4095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4103..4119)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4127..4157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4172..4193)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4481..5069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5243..6100)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6101..6205)
                     /label=AmpR promoter