LOCUS       40924_25441        6308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pIgK3conaLUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     gap             1635..1705
                     /estimated_length=71
     misc_feature    complement(1706..1750)
                     /label=3' UTR of LUC
                     /note="3' UTR of LUC"
     CDS             complement(1754..3403)
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      complement(3538..3701)
                     /label=CONA promoter
                     /note="CONA promoter"
                     /regulatory_class="promoter"
     regulatory      complement(3705..3757)
                     /label=IgK3 enhancer
                     /note="IgK3 enhancer"
                     /regulatory_class="promoter"
     primer_bind     complement(4079..4095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4103..4119)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4127..4157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4172..4193)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4481..5069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5243..6100)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6101..6205)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagnnn cccgggnggt accngagctc
     1621 ngaattcntc tagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnattac aatagctaag aatttcgtca tcgctgaata
     1741 cagttacatt ttacaatttg gactttccgc ccttcttggc ctttatgagg atctctctga
     1801 tttttcttgc gtcgagtttt ccggtaagac ctttcggtac ttcgtccaca aacacaactc
     1861 ctccgcgcaa ctttttcgcg gttgttactt gactggcgac gtaatccacg atctcttttt
     1921 ccgtcatcgt ctttccgtgc tccaaaacaa caacggcggc gggaagttca ccggcgtcat
     1981 cgtcgggaag acctgccacg cccgcgtcga agatgttggg gtgttgtaac aatatcgatt
     2041 ccaattcagc gggggccacc tgatatcctt tgtatttaat taaagacttc aagcggtcaa
     2101 ctatgaagaa gtgttcgtct tcgtcccagt aagctatgtc tccagaatgt agccatccat
     2161 ccttgtcaat caaggcgttg gtcgcttccg gattgtttac ataaccggac ataatcatag
     2221 gtcctctgac acataattcg cctctctgat taacgcccag cgttttcccg gtatccagat
     2281 ccacaacctt cgcttcaaaa aatggaacaa ctttaccgac cgcgcccggt ttatcatccc
     2341 cctcgggtgt aatcagaata gctgatgtag tctcagtgag cccatatcct tgtcgtatcc
     2401 ctggaagatg gaagcgtttt gcaaccgctt ccccgacttc tttcgaaaga ggtgcgcccc
     2461 cagaagcaat ttcgtgtaaa ttagataaat cgtatttgtc aatcagagtg cttttggcga
     2521 agaatgaaaa tagggttggt actagcaacg cactttgaat tttgtaatcc tgaagggatc
     2581 gtaaaaacag ctcttcttca aatctataca ttaagacgac tcgaaatcca catatcaaat
     2641 atccgagtgt agtaaacatt ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag
     2701 tatccggaat gatttgattg ccaaaaatag gatctctggc atgcgagaat ctgacgcagg
     2761 cagttctatg cggaagggcc acacccttag gtaacccagt agatccagag gaattcatta
     2821 tcagtgcaat tgttttgtca cgatcaaagg actctggtac aaaatcgtat tcattaaaac
     2881 cgggaggtag atgagatgtg acgaacgtgt acatcgactg aaatccctgg taatccgttt
     2941 tagaatccat gataataatt ttctggatta ttggtaattt tttttgcacg ttcaaaattt
     3001 tttgcaaccc ctttttggaa acaaacacta cggtaggctg cgaaatgttc atactgttga
     3061 gcaattcacg ttcattataa atgtcgttcg cgggcgcaac tgcaactccg ataaataacg
     3121 cgcccaacac cggcataaag aattgaagag agttttcact gcatacgacg attctgtgat
     3181 ttgtattcag cccatatcgt ttcatagctt ctgccaaccg aacggacatt tcgaagtatt
     3241 ccgcgtacgt gatgttcacc tcgatatgtg catctgtaaa agcaattgtt ccaggaacca
     3301 gggcgtatct cttcatagcc ttatgcagtt gctctccagc ggttccatcc tctagaggat
     3361 agaatggcgc cgggcctttc tttatgtttt tggcgtcttc cattttacca acagtaccgg
     3421 aatgctaatg tcactgcgag cgacattcaa gaatgtaaca caaaggaatt ccaagctttt
     3481 tgcaaaagcc taggcctcca aaaaagcctc ttcactactt ctggaatagg atcgatcctg
     3541 ggtgttgagg agggggagca gcggtgttgg tcacagaccg tcccctctgg gtctgtgatg
     3601 gctgcggagc ctctttcttc cccttttata gaggagcagc cctggctgtg tttgcagaag
     3661 caatgaccta acgctgtttg tccttccttg tttctcaata gggatctggg gaatccccag
     3721 atcatgggga atccccagat catggggaat ccccagatcc tctacgccgg acgcatcgtg
     3781 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     3841 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     3901 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     3961 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     4021 aagggagagc gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatggt
     4081 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4141 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4201 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4921 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5101 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5161 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5221 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5281 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5341 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5401 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5461 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5521 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5581 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5641 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5701 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5761 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5821 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     5881 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5941 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6001 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6061 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6181 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     6241 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     6301 ttcaagaa
//
