piggyBac_RB vector (V005446)

Basic Vector Information

      • Vector Name:
      • piggyBac_RB
      • Length:
      • 10035 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E,

piggyBac_RB vector Vector Map

piggyBac_RB10035 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000AmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 revmini-whiteFRTM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

piggyBac_RB vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_25426       10035 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector piggyBac_RB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Dompe NA, Buchholz R, Ryner L, Miyazaki WY, Kopczynski C, Thibault 
            ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10035)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2004"; volume: "36"; issue: "3"; pages: "283-287"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, South San 
            Francisco, CA 94083, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3100..7236
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    7430..7477
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9641..9657)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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