LOCUS       40924_25426       10035 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector piggyBac_RB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Dompe NA, Buchholz R, Ryner L, Miyazaki WY, Kopczynski C, Thibault 
            ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10035)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2004"; volume: "36"; issue: "3"; pages: "283-287"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, South San 
            Francisco, CA 94083, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3100..7236
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    7430..7477
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9641..9657)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac gctcgcgcga
     2281 cttggtttgc cattctttag cgcgcgtcgc gtcacacagc ttggccacaa tgtggttttt
     2341 gtcaaacgaa gattctatga cgtgtttaaa gtttaggtcg agtaaagcgc aaatcttttt
     2401 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     2461 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     2521 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     2581 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     2641 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2701 gttatagata tcgtgactaa tatataataa aatgggtagt tctttagacg atgagcatat
     2761 cctctctgct cttctgcaaa gcgatgacga gcttgttggt gaggattctg acagtgaaat
     2821 atcagatcac gtaagtgaag atgacgtcca gagcgataca gaagaagcgt ttatagatga
     2881 ggtacatgaa gtgcagccaa cgtcaagcgg tagtgaaata ttagacgaac aaaatgttat
     2941 tgaacaacca ggttcttcat tggcttctaa cagaatcttg accttgccac agaggactat
     3001 tagaggtaag aataaacatt gttggtcaac ttcaaagtcc acgaggcgta gccgagtctc
     3061 tgcactgaac attgtcagat cccccgggct gcaggaattc tagtatgtat gtaagttaat
     3121 aaaacccttt tttggagaat gtagatttaa aaaaacatat ttttttttta ttttttactg
     3181 cactggacat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
     3241 gaagtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
     3301 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
     3361 atctctttcg ccaccgtttg tagcgttacc tagcgtcaat gtccgccttc agttgcactt
     3421 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
     3481 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
     3541 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
     3601 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
     3661 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
     3721 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aatcgcacaa
     3781 acaaatagtg acacgaaaca gattattctg gtagctgtgc tcgctatata agacaatttt
     3841 taagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactaca ttctttttta
     3901 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
     3961 gtataaacct acttcgtagg atacttcgtt ttgttcgggg ttagatgagc ataacgcttg
     4021 tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct
     4081 gcattaacca gggcttcggg caggccaaaa actacggcac gctcctgcca cccagtccgc
     4141 cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca
     4201 atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc
     4261 ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca
     4321 agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca
     4381 attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat
     4441 gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca
     4501 gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa
     4561 ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac
     4621 ggccagggaa cacctgattt tccaggccat ggtgcggatg ccacgacatc tgacctatcg
     4681 gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca
     4741 cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct
     4801 ggcattcgcc tccgaggcac taaccgatcc gccgcttctg atctgcgatg agcccacctc
     4861 cggactggac tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa
     4921 gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga
     4981 caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt
     5041 cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagcat tacattacat
     5101 ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt
     5161 acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca
     5221 agatatgcga caattttgct attagcaaag tagcccggga tatggagcag ttgttggcca
     5281 ccaaaaattt ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct
     5341 ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac
     5401 tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat
     5461 ataacgctta caattcttgg aaacaaattc gctagatttt agttagaatt gcctgattcc
     5521 acacccttct tagttttttt caatgagatg tatagtttat agttttgcag aaaataaata
     5581 aatttcattt aactcgcgaa catgttgaag atatgaatat taatgagatg cgagtaacat
     5641 tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg
     5701 caagtgggcg tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt
     5761 caaaacgtct ttgccacgat aaatgtaagt cttgtttaga atacatttgc atattaataa
     5821 tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt
     5881 tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat
     5941 tgccgaatta ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat
     6001 gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt
     6061 ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat
     6121 ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt
     6181 gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta
     6241 cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg
     6301 cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt
     6361 ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg
     6421 ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat
     6481 ccgaaataac tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc
     6541 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     6601 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     6661 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     6721 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     6781 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     6841 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
     6901 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
     6961 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattctaatt
     7021 taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa
     7081 tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg
     7141 tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat
     7201 ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa
     7261 cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac
     7321 aatgaacagg acctcaattc gcccttgaag atcttgcgac gtgaacagtg agctgtatgt
     7381 gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat
     7441 tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag
     7501 cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctagatct
     7561 tcaagggcga attgggcggc cgcttttgct ggaaacaaac tgtttttgat gatatatgaa
     7621 accaatcaaa actcgctgat tgtgcgtctt tattttttag taaaaaaaaa aaaggaattt
     7681 caagaatgca tcgaaagatt tatattgtca tctgaagtaa atttacttgg atcgcaatcg
     7741 acataggaat tcctacagaa aatacggcct tggccggctt gttctttgat gcgacactgc
     7801 ttaaacgata tccttttgga aacattaatg acgatcccga gaatccgagc gacttcgacg
     7861 atcgcgcgag aagctgcggc tgcggggcga tcgggagcga cgtggcgaac gtgacctgaa
     7921 ataaacgcat tggtaaagaa gaaatgatta cttaaaagct taataaaatt atttaaatca
     7981 gcttagaatg gtaagaagtg tatgtacata cgcgccttac aaagagctgc aaaataggag
     8041 tgttaaatac tgctaaatag ttacttaaac taattaccag ggggaacaaa aatgaaagct
     8101 gtgcaaactg ttttgtttct atgaattaag ctgtgaacca attaagactc gaggccgcct
     8161 taagctgcag taggaagacg aataggtggc ctatggcatt attgtacgga atgataaaca
     8221 ttgcctgcat aaattctttt attatataca gccataatgt cagtagcaag ggagaaaagg
     8281 tccaaagtcg caaaaaattt atgagaaacc tttacatgag cctgacgtca tcgtttatgc
     8341 gtaagcgttt agaagctcct actttgaaga gatatttgcg cgataatatc tctaatattt
     8401 tgccaaatga agtgcctggt acatcagatg acagtactga agagccagta atgaaaaaac
     8461 gtacttactg tacttactgc ccctctaaaa taaggcgaaa ggcaaatgca tcgtgcaaaa
     8521 aatgcaaaaa agttatttgt cgagagcata atattgatat gtgccaaagt tgtttctgac
     8581 tgactaataa gtataatttg tttctattat gtataagtta agctaattac ttattttata
     8641 atacaacatg actgttttta aagtacaaaa taagtttatt tttgtaaaag agagaatgtt
     8701 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     8761 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     8821 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     8881 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     8941 aaataatagt ttctaatttt tttattattc agcctgctgt cgtgaatacc gtatatctca
     9001 acgctgtctg tgagattgtc gtattctagc ctttttagtt tttcgctcat cgacttgata
     9061 ttgtccgaca cattttcgtc gatttgcgtt ttgatcaaag acttgagcag agacacgtta
     9121 atcaactgtt caaattgatc catattaacg atatcaaccc gatgcgtata tggtgcgtaa
     9181 aatatatttt ttaaccctct tatactttgc actctgcgtt aatacgcgtt cgtgtacaga
     9241 cgtaatcatg ttttcttttt tggataaaac tcctactgag tttgacctca tattagaccc
     9301 tcacaagttg caaaacgtgg cattttttac caatgaagaa tttaaagtta ttttaaaaaa
     9361 tttcatcaca gatttaaaga agaaccaaaa attaaattat ttcaacagtt taatcgacca
     9421 gttaatcaac gtgtacacag acgcgtcggc aaaaaacacg cagcccgacg tgttggctaa
     9481 aattattaaa tcaacttgtg ttatagtcac ggatttgccg tccaacgtgt tcctcaaaaa
     9541 gttgaagacc aacaagttta cggacactat taattatttg attttgcccc acttcatttt
     9601 gtgggatcac aattttgtta tattttaaac aaagcttggc actggccgtc gttttacaac
     9661 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9721 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9781 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     9841 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     9901 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     9961 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    10021 caccgaaacg cgcga
//
