pI-SceI vector (V005460)

LOCUS       V005460                 8031 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005460
VERSION     V005460
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Flannagan RS, Linn T, Valvano MA.
  TITLE     A system for the construction of targeted unmarked gene deletions in
            the genus Burkholderia
  JOURNAL   Environ. Microbiol. (2008) In press
   PUBMED   18341581
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Flannagan RS, Linn T, Valvano MA.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8031)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Environ.
            Microbiol. (2008) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2008) Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(54..701)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             807..2003
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        2243..2271
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2612..3304
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             3326..3340
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     terminator      3568..3654
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3746..3773
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        complement(4628..4730)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      6123..6892
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             6893..7552
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"