LOCUS       V005460                 8031 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005460
VERSION     V005460
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Flannagan RS, Linn T, Valvano MA.
  TITLE     A system for the construction of targeted unmarked gene deletions in
            the genus Burkholderia
  JOURNAL   Environ. Microbiol. (2008) In press
   PUBMED   18341581
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Flannagan RS, Linn T, Valvano MA.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8031)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Environ.
            Microbiol. (2008) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2008) Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(54..701)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             807..2003
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        2243..2271
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2612..3304
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             3326..3340
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     terminator      3568..3654
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3746..3773
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        complement(4628..4730)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      6123..6892
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             6893..7552
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 atcgatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
       61 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
      121 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
      181 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
      241 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
      301 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
      361 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
      421 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
      481 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
      541 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
      601 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
      661 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
      721 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
      781 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga
      841 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
      901 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
      961 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     1021 ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc atcatggcga
     1081 cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     1141 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     1201 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     1261 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     1321 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     1381 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     1441 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     1501 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     1561 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     1621 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     1681 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     1741 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     1801 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     1861 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     1921 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
     1981 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggaaacgtgg ccaatatgga
     2041 caacttcttc gcatcgatct gcacttgaac gtgtggccta agcgatatct taggatctcc
     2101 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     2161 ccacgatgcg tccggcgtag aggatctgct catgtttgac agcttactcg agtatgctag
     2221 cgatgagctc gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa
     2281 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2341 cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc
     2401 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2461 tgttacgcag cagggcagtc gccctaaaac aaagttaggc agccgttgtg ctggtgcttt
     2521 ctgatagttg ttgtggggta ggcagtcaga gctcgatttg cttgtcgcca taatagattc
     2581 acaagaagga ttcggaattc tacatatgca tcaaaaaaac caggtaatga acctgggtcc
     2641 gaactctaaa ctgctgaaag aatacaaatc ccagctgatc gaactgaaca tcgaacagtt
     2701 cgaagcaggt atcggtctga tcctgggtga tgcttacatc cgttctcgtg atgaaggtaa
     2761 aacctactgt atgcagttcg agtggaaaaa caaagcatac atggaccacg tatgtctgct
     2821 gtacgatcag tgggtactgt ccccgccgca caaaaaagaa cgtgttaacc acctgggtaa
     2881 cctggtaatc acctggggcg cccagacttt caaacaccaa gctttcaaca aactggctaa
     2941 cctgttcatc gttaacaaca aaaaaaccat cccgaacaac ctggttgaaa actacctgac
     3001 cccgatgtct ctggcatact ggttcatgga tgatggtggt aaatgggatt acaacaaaaa
     3061 ctctaccaac aaatcgatcg tactgaacac ccagtctttc actttcgaag aagtagaata
     3121 cctggttaag ggtctgcgta acaaattcca actgaactgt tacgtaaaaa tcaacaaaaa
     3181 caaaccgatc atctacatcg attctatgtc ttacctgatc ttctacaacc tgatcaaacc
     3241 gtacctgatc ccgcagatga tgtacaaact gccgaacact atctcctccg aaactttcct
     3301 gaaataatct agagactaca aggacgacga cgacgacaag tgacgacctg caggcatgca
     3361 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     3421 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     3481 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     3541 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3601 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3661 ggagcggatt tgaacgctgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     3721 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     3781 ctacaaactc ttccactcac tacagcagag ccatttaaac aacatcccct ccccctttcc
     3841 accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc
     3901 aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctgaggt ctgcctcgtg
     3961 aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga
     4021 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt
     4081 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag
     4141 caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca
     4201 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     4261 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     4321 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     4381 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     4441 aagcgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gctaaaaagg
     4501 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     4561 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     4621 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     4681 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     4741 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     4801 ctgcgaagtg atcttccgtc acaggtattt attcgaagac gaaagggcct cgtgatacgc
     4861 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4921 cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc ctgtttctgg cgctggactt
     4981 cccgctgttc cgtcagcagc ttttcgccca cggccttgat gatcgcggcg gccttggcct
     5041 gcatatcccg attcaacggc cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt
     5101 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
     5161 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
     5221 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
     5281 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
     5341 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
     5401 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
     5461 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
     5521 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
     5581 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
     5641 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
     5701 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
     5761 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
     5821 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
     5881 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
     5941 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
     6001 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
     6061 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     6121 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     6181 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     6241 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     6301 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     6361 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     6421 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     6481 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     6541 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     6601 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     6661 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     6721 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     6781 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     6841 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     6901 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     6961 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     7021 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     7081 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     7141 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     7201 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     7261 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     7321 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     7381 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     7441 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     7501 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     7561 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     7621 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     7681 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     7741 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     7801 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     7861 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     7921 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     7981 gcgtttaagt cgaccaataa ctctacgggg tctgacgctc agtggaacga a
//
