pHyVec16 vector (V005469)

LOCUS       40924_25236        5373 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec16, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5373)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2014) Department of Biological Chemistry, University of 
            California, Irvine, D240 Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    400..499
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     896..912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        922..940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      950..1853
                     /label=hydra actin promoter
                     /note="hydra actin promoter"
                     /regulatory_class="promoter"
     CDS             1998..2672
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    2676..3171
                     /label=hydra actin 3' flanking sequence
                     /note="hydra actin 3' flanking sequence"
     regulatory      2807..2812
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(3185..3203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3224..3240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3248..3264)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3272..3302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3317..3338)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3626..4214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4388..5245)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5246..5350)
                     /label=AmpR promoter