LOCUS       40924_25236        5373 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec16, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5373)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2014) Department of Biological Chemistry, University of 
            California, Irvine, D240 Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    400..499
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     896..912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        922..940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      950..1853
                     /label=hydra actin promoter
                     /note="hydra actin promoter"
                     /regulatory_class="promoter"
     CDS             1998..2672
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    2676..3171
                     /label=hydra actin 3' flanking sequence
                     /note="hydra actin 3' flanking sequence"
     regulatory      2807..2812
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(3185..3203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3224..3240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3248..3264)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3272..3302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3317..3338)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3626..4214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4388..5245)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5246..5350)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccggatcca aggtaccttc tcgaggtacc tgcagtactg acggacacac cgaagccccg
      361 gcggcaaccc tcagcggatg ccccggggct tcacgttttc ccaggtcaga agcggttttc
      421 gggagtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc gtagggtcgc
      481 cgacatgaca caaggggttg tgaccggggt ggacacgtac gcgggtgctt acgaccgtca
      541 gtcgcgcgag cgcgatctag actatcgata ggatatctca gatctaggtc gacgagcccc
      601 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
      661 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
      721 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
      781 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      841 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      901 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggt tttaagccga
      961 gatgcaggat tctgaatgaa aaagaaaaaa agaagtctcg gtagagtaaa agtgatcggt
     1021 ttgcaactgt aaaatttatt gaagtaccaa taattttatt taaaataaaa ctgaaatata
     1081 aagttaaagt tgctgttcta taagtttacc gaattttaaa accattgtaa cgctagagta
     1141 atatttgagt ctactaagtt agtccccgca ctttttaatc aagcaataaa tacccaaact
     1201 ttgcttattc aaatcaataa accaatatat ctcttaaaat aaagtaaaaa cttctgaaat
     1261 tctataaaaa aaaatttaat ttcgaaatat caaatgtaac ttcaacaccg cactattttc
     1321 ttttaaacaa ctgatatagt aattacttct caaaaacgtt atctcaaggt ttgtgatgta
     1381 cttaaaacca ctcctatttt gttacgcgtt taaaaaagca aacataagtt ggtttctatt
     1441 gatgaatgag aacatatttc atttaaagtt aaaatcctac cagtggtttc actgtacgta
     1501 aacaccgtca aaaaaacagg aacgttttta aagattaata attgaagtaa aaaaaattta
     1561 ataccggggg ttaaaaaaat cttttaaaat aattataaat atatatatta aaatttataa
     1621 atttttaaac acatttaaaa tatatattaa gtataataaa agtaatatta taaaaaaaaa
     1681 tttaatttta taattatttt tattaaattt ataaataata ggtaaaactt acatatccgt
     1741 tttatttttt cttaataaaa taacgcgtgc aaatttttgt ccatataaag accttttcga
     1801 acaataactt ttttgcttag ccgttttttt tcttatatgg tcaaaaaagc gctcaagcga
     1861 ttcaccataa aaagcgcaat tagttcagcg ttcgttattc agaagcttca gctttgcttg
     1921 atactcagct cttctctttt taaacaaaac acttaatcaa aatggccgat gatgaagttg
     1981 ccgccctcgc tgcagaaatg gcttcatcag aaaatgttat tacagaattt atgagattta
     2041 aagttagaat ggaaggtaca gttaatggac atgaatttga aattgaggga gaaggagaag
     2101 gtagaccata tgaaggacat aatacagtta aattaaaggt tacaaaagga ggaccattac
     2161 catttgcgtg ggatatttta tcaccacaat ttcaatatgg atcaaaggtt tacgttaaac
     2221 atccagctga tattccagat tataaaaaat tatcttttcc agaaggattt aaatgggaaa
     2281 gagttatgaa ttttgaagat ggtggagttg ctacagttac acaagattca tcattacaag
     2341 atggttgttt tatttataaa gttaaattta ttggggttaa ttttccatca gacggaccag
     2401 ttatgcaaaa aaaaacaatg ggatgggaag ctagtacaga aagattatat ccaagagacg
     2461 gggttttaaa gggagaaaca cataaagctc taaaattaaa ggacggagga cattatttag
     2521 ttgaatttaa atcaatttat atggctaaaa aaccagttca attaccagga tattattatg
     2581 tagatgctaa gttagatatt acatcacata atgaagatta tacaattgtt gaacaatatg
     2641 aaagaacaga aggtagacat catttatttt tatagcattc gtagaattca caattcgatt
     2701 atatttatac tggactattt ttacatctgt tcggttattt tcacatttat ttttctatat
     2761 atatcttata aacgttttaa aacccatgta atttttgtta agctgtaata taaaagacgt
     2821 cctaacaaac ttcttttatt actgaatttc ctttaattat aataaataac aagttttaaa
     2881 ataaattcag gcaattaagg cgctcctgag gtactaaaat taatgtaaac atttaaaatt
     2941 aacttggatg gtcttaagta ctgtactcgt gattttgtta tactttatta ttagaaaagt
     3001 cgtctattaa ctttttgttc cttaatttac ttgattaaat tgtcgcttaa tttatcaaat
     3061 caggttttgc gcgttatttt agagaaaaac ttattagaaa aatgaataag caaagtttag
     3121 gctaacatgt ttttttatta ttttaaatag ttcaagtcaa tgacgtataa accagctttt
     3181 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     3241 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3301 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3361 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3421 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4381 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4441 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4501 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4561 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4621 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4681 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4741 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4801 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4861 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4921 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4981 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5041 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5101 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5161 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5221 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5281 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5341 gggttccgcg cacatttccc cgaaaagtgc cac
//
