pHyVec14 vector (V005470)

LOCUS       40924_25231        5153 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5153)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2014) Department of Biological Chemistry, University of 
            California, Irvine, D240 Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    304..373
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      730..1633
                     /label=derived from Hydra actin gene
                     /note="derived from Hydra actin gene"
                     /regulatory_class="promoter"
     CDS             1778..2452
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    2456..2951
                     /label=3' flanking sequence from Hydra actin
                     /note="3' flanking sequence from Hydra actin"
     regulatory      2587..2592
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(2965..2983)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3004..3020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3028..3044)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3052..3082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3097..3118)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3406..3994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4168..5025)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5026..5130)
                     /label=AmpR promoter