LOCUS       40924_25231        5153 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5153)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2014) Department of Biological Chemistry, University of 
            California, Irvine, D240 Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    304..373
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      730..1633
                     /label=derived from Hydra actin gene
                     /note="derived from Hydra actin gene"
                     /regulatory_class="promoter"
     CDS             1778..2452
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    2456..2951
                     /label=3' flanking sequence from Hydra actin
                     /note="3' flanking sequence from Hydra actin"
     regulatory      2587..2592
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(2965..2983)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3004..3020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3028..3044)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3052..3082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3097..3118)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3406..3994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4168..5025)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5026..5130)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccggatcca aggtaccttc tcgagttact agtagtctag actatcgata ggatatctca
      361 gatctaggtc gacgagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
      541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      661 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
      721 gcgaattggt tttaagccga gatgcaggat tctgaatgaa aaagaaaaaa agaagtctcg
      781 gtagagtaaa agtgatcggt ttgcaactgt aaaatttatt gaagtaccaa taattttatt
      841 taaaataaaa ctgaaatata aagttaaagt tgctgttcta taagtttacc gaattttaaa
      901 accattgtaa cgctagagta atatttgagt ctactaagtt agtccccgca ctttttaatc
      961 aagcaataaa tacccaaact ttgcttattc aaatcaataa accaatatat ctcttaaaat
     1021 aaagtaaaaa cttctgaaat tctataaaaa aaaatttaat ttcgaaatat caaatgtaac
     1081 ttcaacaccg cactattttc ttttaaacaa ctgatatagt aattacttct caaaaacgtt
     1141 atctcaaggt ttgtgatgta cttaaaacca ctcctatttt gttacgcgtt taaaaaagca
     1201 aacataagtt ggtttctatt gatgaatgag aacatatttc atttaaagtt aaaatcctac
     1261 cagtggtttc actgtacgta aacaccgtca aaaaaacagg aacgttttta aagattaata
     1321 attgaagtaa aaaaaattta ataccggggg ttaaaaaaat cttttaaaat aattataaat
     1381 atatatatta aaatttataa atttttaaac acatttaaaa tatatattaa gtataataaa
     1441 agtaatatta taaaaaaaaa tttaatttta taattatttt tattaaattt ataaataata
     1501 ggtaaaactt acatatccgt tttatttttt cttaataaaa taacgcgtgc aaatttttgt
     1561 ccatataaag accttttcga acaataactt ttttgcttag ccgttttttt tcttatatgg
     1621 tcaaaaaagc gctcaagcga ttcaccataa aaagcgcaat tagttcagcg ttcgttattc
     1681 agaagcttca gctttgcttg atactcagct cttctctttt taaacaaaac acttaatcaa
     1741 aatggccgat gatgaagttg ccgccctcgc tgcagaaatg gcttcatcag aaaatgttat
     1801 tacagaattt atgagattta aagttagaat ggaaggtaca gttaatggac atgaatttga
     1861 aattgaggga gaaggagaag gtagaccata tgaaggacat aatacagtta aattaaaggt
     1921 tacaaaagga ggaccattac catttgcgtg ggatatttta tcaccacaat ttcaatatgg
     1981 atcaaaggtt tacgttaaac atccagctga tattccagat tataaaaaat tatcttttcc
     2041 agaaggattt aaatgggaaa gagttatgaa ttttgaagat ggtggagttg ctacagttac
     2101 acaagattca tcattacaag atggttgttt tatttataaa gttaaattta ttggggttaa
     2161 ttttccatca gacggaccag ttatgcaaaa aaaaacaatg ggatgggaag ctagtacaga
     2221 aagattatat ccaagagacg gggttttaaa gggagaaaca cataaagctc taaaattaaa
     2281 ggacggagga cattatttag ttgaatttaa atcaatttat atggctaaaa aaccagttca
     2341 attaccagga tattattatg tagatgctaa gttagatatt acatcacata atgaagatta
     2401 tacaattgtt gaacaatatg aaagaacaga aggtagacat catttatttt tatagcattc
     2461 gtagaattca caattcgatt atatttatac tggactattt ttacatctgt tcggttattt
     2521 tcacatttat ttttctatat atatcttata aacgttttaa aacccatgta atttttgtta
     2581 agctgtaata taaaagacgt cctaacaaac ttcttttatt actgaatttc ctttaattat
     2641 aataaataac aagttttaaa ataaattcag gcaattaagg cgctcctgag gtactaaaat
     2701 taatgtaaac atttaaaatt aacttggatg gtcttaagta ctgtactcgt gattttgtta
     2761 tactttatta ttagaaaagt cgtctattaa ctttttgttc cttaatttac ttgattaaat
     2821 tgtcgcttaa tttatcaaat caggttttgc gcgttatttt agagaaaaac ttattagaaa
     2881 aatgaataag caaagtttag gctaacatgt ttttttatta ttttaaatag ttcaagtcaa
     2941 tgacgtataa accagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     3001 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3061 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3841 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4141 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4201 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4261 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4321 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4381 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4441 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4501 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4561 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4621 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4681 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4741 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4801 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4861 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4921 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4981 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
