pHsCXW vector (V005488)

LOCUS       V005488                 7207 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005488
VERSION     V005488
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Leander Johansen J, Dago L, Tornoe J, Rosenblad C, Kusk P.
  TITLE     A new versatile and compact lentiviral vector
  JOURNAL   Mol. Biotechnol. 29 (1), 47-56 (2005)
   PUBMED   15668519
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Johansen J, Tornoe J, Rosenblad C, Dago L, Kusk P.
  TITLE     Improved lentiviral transfer vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7207)
  AUTHORS   Kusk P, Johansen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2003) NsGene A/S, Baltorpvej 154, Ballerup
            DK-2750, Denmark
REFERENCE   4  (bases 1 to 7207)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7207)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biotechnol."; date: "2005"; volume: "29"; issue: "1"; pages: "47-56"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-NOV-2003) NsGene A/S, Baltorpvej 154, Ballerup DK-2750, Denmark"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1299..1532
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1717..1761
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1910..1951
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        2040..2343
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2344..2547
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    2618..2671
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     promoter        complement(2675..2693)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2721..3309
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3514..3746
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      complement(4778..5366)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5540..6397)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6398..6502)
                     /label="AmpR promoter"
     polyA_signal    6906..7040
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"