LOCUS       V005488                 7207 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005488
VERSION     V005488
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Leander Johansen J, Dago L, Tornoe J, Rosenblad C, Kusk P.
  TITLE     A new versatile and compact lentiviral vector
  JOURNAL   Mol. Biotechnol. 29 (1), 47-56 (2005)
   PUBMED   15668519
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Johansen J, Tornoe J, Rosenblad C, Dago L, Kusk P.
  TITLE     Improved lentiviral transfer vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7207)
  AUTHORS   Kusk P, Johansen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2003) NsGene A/S, Baltorpvej 154, Ballerup
            DK-2750, Denmark
REFERENCE   4  (bases 1 to 7207)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7207)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biotechnol."; date: "2005"; volume: "29"; issue: "1"; pages: "47-56"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-NOV-2003) NsGene A/S, Baltorpvej 154, Ballerup DK-2750, Denmark"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1299..1532
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1717..1761
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1910..1951
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        2040..2343
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2344..2547
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    2618..2671
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     promoter        complement(2675..2693)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2721..3309
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3514..3746
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      complement(4778..5366)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5540..6397)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6398..6502)
                     /label="AmpR promoter"
     polyA_signal    6906..7040
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gataagcttg ggagttccgc
     2041 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2101 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2161 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2221 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2281 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2341 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2401 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2461 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2521 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2581 catccacgct gttttgacct ccatagaaga caccgactct agaggatccc gtacgccatg
     2641 gactagtccc ggggtcgaca cgcgtctcga gcgccctata gtgagtcgta ttattcgagc
     2701 tcaagcttcg aattcccgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2761 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2821 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2881 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2941 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3001 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3061 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     3121 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3181 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3241 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3301 cctccccgca tcgggaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     3361 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     3421 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     3481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3541 gacaagactg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     3781 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag
     3841 caggaattga actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc
     3901 accagagata ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt
     3961 aagtttacta acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact
     4021 attctgacag atattaatta atcctcaaat atcataagag atgattacta ttatccccat
     4081 ttaacacaag aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca
     4141 tccgttagtt acgactctat gatcttctga cacaaattcc atttactcct caccctatga
     4201 ctcagtcgaa tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat
     4261 agtaaatact gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag
     4321 aagagaagtg aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt
     4381 tagaaaggga ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca
     4441 atataacctt aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt
     4501 attgaagatt atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg
     4561 tatcattaga gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta
     4621 acacaagtag aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt
     4681 agaaagagat aacaacaatt tgaattattg aaagtaacat gtgagcaaaa ggccagcaaa
     4741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     6541 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     6601 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6661 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6721 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6781 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     6841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     6901 gccagaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7021 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     7081 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     7141 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     7201 tagtagt
//
