pHRS-9 vector (V005492)

LOCUS       40924_24997        8530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHRS-9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2003) Department of Biochemistry and Molecular 
            Biology, Universidade Federal do Parana - UFPR, Centro Politecnico, 
            Curitiba, PR 81531-990, Brazil
REFERENCE   2  (bases 1 to 8530)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2003) Department of Biochemistry and Molecular Biology, 
            Universidade Federal do Parana - UFPR, Centro Politecnico, Curitiba,
            PR 81531-990, Brazil"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..28
                     /label=derived from suicide plasmid pSUP202
                     /note="derived from suicide plasmid pSUP202"
     misc_feature    29..838
                     /note="RS-alpha repetitive sequence from Bradyrhizobium 
                     japonicum CPAC7"
     misc_feature    338..412
                     /label=SstI-BamHI-BglII multiple cloning site
                     /note="SstI-BamHI-BglII multiple cloning site"
     CDS             896..2083
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             2727..2915
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3020..3160
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3346..3934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4108..4965)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4966..5070)
                     /label=AmpR promoter
     promoter        5273..5375
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5376..6032
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     oriT            6916..7025
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             7058..7426
                     /label=traJ
                     /note="oriT-recognizing protein"