LOCUS       40924_24997        8530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHRS-9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2003) Department of Biochemistry and Molecular 
            Biology, Universidade Federal do Parana - UFPR, Centro Politecnico, 
            Curitiba, PR 81531-990, Brazil
REFERENCE   2  (bases 1 to 8530)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2003) Department of Biochemistry and Molecular Biology, 
            Universidade Federal do Parana - UFPR, Centro Politecnico, Curitiba,
            PR 81531-990, Brazil"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..28
                     /label=derived from suicide plasmid pSUP202
                     /note="derived from suicide plasmid pSUP202"
     misc_feature    29..838
                     /note="RS-alpha repetitive sequence from Bradyrhizobium 
                     japonicum CPAC7"
     misc_feature    338..412
                     /label=SstI-BamHI-BglII multiple cloning site
                     /note="SstI-BamHI-BglII multiple cloning site"
     CDS             896..2083
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             2727..2915
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3020..3160
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3346..3934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4108..4965)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4966..5070)
                     /label=AmpR promoter
     promoter        5273..5375
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5376..6032
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     oriT            6916..7025
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             7058..7426
                     /label=traJ
                     /note="oriT-recognizing protein"
ORIGIN
        1 aggccatgtt tgacagctta tcatcgataa gcttgcggtt cggaaaagnc gcggtgacgc
       61 tgttcatgaa atcgagaaac tcgacgcggc gccggcgttt tgaatgggtc gcgatgatct
      121 ttccggtggc gacttcgagc gccgcaaaca atgttgtggt gccatgccgc ttgtaatcgt
      181 ggctttggcc ggttaaggcg cggccantgg gcaacttcag ataaccctgc gctcgctcca
      241 aagcctggat cgagggcttc tcgtccacgc acagcacaat ggccttcgcc ggcggcggac
      301 atagaggccg acaacatcgg cggctttggc cgaaagttcg ggtcgttgct ctcgcaccag
      361 gacttgcgag ccaccaggtc aatacttgag ctctcgagga tccgccagat ctattggaca
      421 tcgcccagcg cctcggccag cagggggccg gtccagcgcg caaacccttg cggtggcggc
      481 ttatccagca gcttcagaat ccgcttgtcg gtcgtcttcg tatagatcgg ctgcttgcca
      541 ggccgcggct tgtcttgcag cccttcaagg ccatggtcgg catagcgatg ccgccaaagg
      601 ctgacaatcc gcggctggac cccaacttcc ttggcgatcg accgggtgct gcgcccatcc
      661 gccgccaaca gaactatccg cgcccgcttc aaatcgcgct gcaacgtcac cggtgagcga
      721 cagaccgcct caagcacctt gcgatctttc ctcgaaaggt ggacttctct tgcttcgggt
      781 atcatcccga ccttgaatca cgactcacgt tccaagaaaa gctgacctgc aggcatgcaa
      841 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
      901 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
      961 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     1021 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     1081 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     1141 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     1201 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     1261 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     1321 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     1381 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     1441 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     1501 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     1561 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     1621 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     1681 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     1741 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     1801 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     1861 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     1921 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
     1981 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     2041 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     2101 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     2161 gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc
     2221 cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     2281 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     2341 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     2401 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     2461 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     2521 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     2581 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     2641 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     2701 aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg
     2761 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     2821 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     2881 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2941 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3001 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     3061 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     3121 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3781 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4081 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4141 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4201 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4261 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4321 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4381 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     4441 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4501 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4561 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4621 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4681 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     4741 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4801 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4861 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4921 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5041 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5101 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5161 tcgtcttcga ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg
     5221 tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc
     5281 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     5341 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     5401 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     5461 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5521 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5581 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5641 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5701 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5761 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5821 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5881 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     5941 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     6001 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     6061 cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     6121 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
     6181 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     6241 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcctt
     6301 tttgtccggt gttgggttga aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc
     6361 agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc
     6421 cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt gccgcctggc
     6481 gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc gtttcccgca tgtgctccca
     6541 aatggtgacg agcgcatagc cggacgctaa cgccgcctcg acatccgccc tcaccgccag
     6601 gaacgcaacc gcagcctcat cacgccggcg cttcttggcc gcgcgggatt caacccactc
     6661 ggccagctcg tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc agttcagtaa
     6721 tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg
     6781 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     6841 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     6901 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     6961 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     7021 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
     7081 caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga
     7141 ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg
     7201 ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg
     7261 cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc
     7321 gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga
     7381 gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc
     7441 gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga
     7501 agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc
     7561 tgggtcacgt gcgcgtcacg aactgcgagg caaacaccct gcccgctgtc atggccgagg
     7621 tgatggcgac ccagcacggc aacacccgtt ccgaggccga caagacctat cacctgctgg
     7681 ttagcttccg cgcgggagag aagcccgacg cggagacgtt gcgcgcgatt gaggaccgca
     7741 tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag tgccgtgcat cacgacaccg
     7801 acaacctgca catccatatc gccatcaaca agattcaccc gacccgaaac accatccatg
     7861 agccgtatcg ggcctaccgc gccctcgctg acctctgcgc gacgctcgaa cgggactacg
     7921 ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc cgagaaccgc gcgaacgaca
     7981 tggagcggca cgcgggcgtg gaaagcctgg tcggctggat catttattct gcctcccaga
     8041 gcctgataaa aacggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     8101 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     8161 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     8221 cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     8281 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     8341 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     8401 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     8461 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     8521 cgcaagcgac
//
