phPK14H vector (V005521)

LOCUS       40924_24822        6815 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Keratinocyte expression vector phPK14H, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     A recombinant plasmid for specific expression of recombinant protein
            in human kertinocytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Keratinocyte specific-expression vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2006) Molecular Genetics, The National Institute 
            for Genetic Engineering and Biotechnology, Blvd. Pjoohesh. km-17 
            Tehran-karaj Highway, Tehran, Tehran 14155-6343, Iran
REFERENCE   4  (bases 1 to 6815)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2006) Molecular Genetics, The National Institute for Genetic
            Engineering and Biotechnology, Blvd. Pjoohesh. km-17 Tehran-karaj 
            Highway, Tehran, Tehran 14155-6343, Iran"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(260..277)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    2258..2363
                     /label=multiple cloning site (MCS)
                     /note="multiple cloning site (MCS)"
     promoter        complement(2367..2385)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2411..2635
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2681..3109
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3123..3453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3520..4311
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4488..4621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4658..4674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4682..4698)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4706..4736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4751..4772)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5060..5648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5822..6679)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6680..6784)
                     /label=AmpR promoter
     promoter        6815
                     /label=K14
                     /note="Human keratin 14 promoter"