LOCUS       40924_24822        6815 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Keratinocyte expression vector phPK14H, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     A recombinant plasmid for specific expression of recombinant protein
            in human kertinocytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Keratinocyte specific-expression vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6815)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2006) Molecular Genetics, The National Institute 
            for Genetic Engineering and Biotechnology, Blvd. Pjoohesh. km-17 
            Tehran-karaj Highway, Tehran, Tehran 14155-6343, Iran
REFERENCE   4  (bases 1 to 6815)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2006) Molecular Genetics, The National Institute for Genetic
            Engineering and Biotechnology, Blvd. Pjoohesh. km-17 Tehran-karaj 
            Highway, Tehran, Tehran 14155-6343, Iran"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(260..277)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    2258..2363
                     /label=multiple cloning site (MCS)
                     /note="multiple cloning site (MCS)"
     promoter        complement(2367..2385)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2411..2635
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2681..3109
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3123..3453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3520..4311
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4488..4621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4658..4674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4682..4698)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4706..4736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4751..4772)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5060..5648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5822..6679)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6680..6784)
                     /label=AmpR promoter
     promoter        6815
                     /label=K14
                     /note="Human keratin 14 promoter"
ORIGIN
        1 gacggatcgg gagatctgct agggttctgg tgttggtgcg tggggttggg gtgggactgc
       61 agaagtgcct tttaagatta tgtgattgac tgatctgtca ttggttccct gccatcttta
      121 tcttttggat tcccctcgga ggagggggag gaaggagttt cttttgggtt ttattgaatc
      181 aaatgaaagg gaaagtagag gtgttcctat gtcccgggct ccggagcttc tattcctgat
      241 ccctgcataa gaaggagaca tggtggtggt ggtggtgggt gggggtggtg gggcacagag
      301 gaagccgata ctgggctctg caccccattc ccgctcccag atccctctgg acacagcatt
      361 tttctccagt gagcacagcc tccccttgcc ccacagccaa cagcaacatg cctcccaaca
      421 aaagcatctg tccctcagcc aaaacccctg ttgcctctct ctggggaaat tgtagggctg
      481 ggccagggtg gggggaccat tctctgcagg gagattagga gtktctgtca ggggcgggtg
      541 gagcggggtg gggccctggc ttactcacat ccttgagagt cctttgctgg cagatttggg
      601 gagcccacag ctcagatgtc tgtctcagca ttgtcttcca agctcctagg ccacagtagt
      661 ggggggctcc cttctctggc ttcttctttg gtgacagtca aggtggggtt gggggtgaca
      721 gagggtcctg cttctcttct aggagcagtt gatcccagga agagcattgg agcctccagc
      781 aggggctgtt ggggcctgtc tgaggagata ggatgcgtca ggcagcccca gacacgatca
      841 cattcctctc aacatgcctg ccggggtctg tggagccgag gggctgatgg gagggtgggg
      901 tgggggccgg aagggtttgc tttgggaggt tgtctgggag attgctgaag ttttgatata
      961 cacacctcca aagcaggacc aagtggactc ctacccctga cccttggggc ttcaggagtc
     1021 agggaccctc gtgtccacct cagccttgcc cttggcacag cccagctcca ctccagcctc
     1081 tactcctccc cagaacatct cctgggccag ttccacaagg ggctcaaacg agggcacctg
     1141 agctgcccac actagggatg ttctgggggt ctgagaagat atctggggct ggaagaataa
     1201 aaggcccccc taggcctgtt cctggatgca gctccagcca ctttggggct aagcctgggc
     1261 tataacaatg ccaacgaggc ctcttgccat actcggttta caaaaccctt tcacatacat
     1321 tgtcgcattg gattctcaga gctgactgca ctaagcagaa tagatggtat gactcccact
     1381 ttgcagatga gaacactgag gctcagagaa gtgccaagcc ctgggtcaca gaggcgtaaa
     1441 tggcagagcc aggacccacc tgactccagg ctgtttcctg gcctccatga ggccacctgc
     1501 cctatggtgt ggtggatgtg agatcctcac catagggagg agattagggt ctgtgctcag
     1561 ggctggggag agctgcctgg atttctcttt gatggggatg ttggggtggg aatcacgata
     1621 cacctgacta gctgggtgta tttcagggat gggacagact tctcagcaca gcacggcagg
     1681 tcaggcctgg gagggccccc cagacctcct tgtctctaat agagggtcat ggtgagggag
     1741 gcctgtctgt gcccaaggtg accttgccat gccggtgctt tccagccggg tatccatccc
     1801 ctgcagcagc aggcttcctc tacgtggatg ttaaaggccc attcagttca tggagagcta
     1861 gcaggtaact aggtttaagg tgcagaggcc ctgctctctg tcaccctggc taagcccagt
     1921 gcgtgggttc ctgagggctg ggactcccag ggtccgatgg gaaagtgtag cctgcaggcc
     1981 cacacctccc cctgtgaatc acgcctggcg ggacaagaaa gcccaaaaca ctccaaacaa
     2041 tgagtttcca gtaaaatatg acagacatga tgaggcggat gagaggaggg acctggctgg
     2101 gagttggcgc tagcctgtgg gtgatgaaag ccaaggggaa tggaaagtgc cagacccgcc
     2161 ccctacccat gagtataaag cactcgcatc cctttccaat ttacccgagc accttctctt
     2221 cactcagcca actgctgcgt cgctcacctc cctcctcaag cttggtaccg agctcggatc
     2281 cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac tggcggccgc
     2341 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     2401 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2461 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2521 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2581 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2641 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     2701 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2761 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2821 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2881 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2941 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3001 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
     3061 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     3121 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa
     3181 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3361 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     3421 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     3481 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3541 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3601 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3661 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     3721 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3781 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3841 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3901 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3961 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4021 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4081 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4141 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4201 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4261 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4321 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4381 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4441 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     4501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     4621 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4681 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4741 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4801 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4861 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5041 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5221 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     5281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5461 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     5521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5701 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5761 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5821 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5881 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5941 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6001 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6061 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6121 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6181 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6241 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6301 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6361 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6421 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6481 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6541 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6601 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6661 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6721 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6781 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
