pHomeT-1-R vector (V005526)

LOCUS       40924_24787        9545 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PiggyBac cloning vector pHomeT-1-R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Windbichler N, Menichelli M, Papathanos PA, Thyme SB, Li H, Ulge UY,
            Hovde BT, Baker D, Monnat RJ Jr., Burt A, Crisanti A.
  TITLE     A synthetic homing endonuclease-based gene drive system in the human
            malaria mosquito
  JOURNAL   Nature 473 (7346), 212-215 (2011)
  PUBMED    21508956
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Windbichler N.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Imperial College London, Imperial College 
            Road, London SW7 2AZ, England
REFERENCE   3  (bases 1 to 9545)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "473"; issue: "7346"; pages: "212-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2010) Imperial College London, Imperial College Road, London
            SW7 2AZ, England"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..736
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    862..983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1051..3628
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3640..4317
                     /label=DsRed1
                     /note="wild-type DsRed"
     polyA_signal    4488..4918
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(6038..6107)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     primer_bind     complement(6143..6159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6167..6183)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6191..6221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6236..6257)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6545..7133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7307..8164)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8165..8269)
                     /label=AmpR promoter
     regulatory      9245..9505
                     /gene="3xP3"
                     /regulatory_class="promoter"
     gene            9245..9505
                     /gene="3xP3"
                     /label=3xP3