LOCUS       40924_24787        9545 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PiggyBac cloning vector pHomeT-1-R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Windbichler N, Menichelli M, Papathanos PA, Thyme SB, Li H, Ulge UY,
            Hovde BT, Baker D, Monnat RJ Jr., Burt A, Crisanti A.
  TITLE     A synthetic homing endonuclease-based gene drive system in the human
            malaria mosquito
  JOURNAL   Nature 473 (7346), 212-215 (2011)
  PUBMED    21508956
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Windbichler N.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Imperial College London, Imperial College 
            Road, London SW7 2AZ, England
REFERENCE   3  (bases 1 to 9545)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "473"; issue: "7346"; pages: "212-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2010) Imperial College London, Imperial College Road, London
            SW7 2AZ, England"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..736
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    862..983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1051..3628
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3640..4317
                     /label=DsRed1
                     /note="wild-type DsRed"
     polyA_signal    4488..4918
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(6038..6107)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     primer_bind     complement(6143..6159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6167..6183)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6191..6221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6236..6257)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6545..7133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7307..8164)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8165..8269)
                     /label=AmpR promoter
     regulatory      9245..9505
                     /gene="3xP3"
                     /regulatory_class="promoter"
     gene            9245..9505
                     /gene="3xP3"
                     /label=3xP3
ORIGIN
        1 tagggataac agggtaatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
       61 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      181 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      421 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      481 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      601 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      721 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
      781 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      841 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      961 tccaaactca tcaatgtatc ttaaagctta tcgatacgcg tacggcgcgc ctaggccggc
     1021 cgaattcaaa tgatacttct aaaaaaaaat catgaatggc atcaactctg aatcaaatct
     1081 ttgcagatgc acctacttct catttccact gtcacatcat ttttccagat ctcgctgcct
     1141 gttatgtggc ccacaaacca agacacgttt tatggccatt aaagctggct gatcgtcgcc
     1201 aaacaccaaa tacataatga atatgtacac attcgagaaa gaagcgatca aagaagcgtc
     1261 ttcgggcgga gtaggagaat gcggaggaga aggagaacga gctgatctag tatctctcca
     1321 caatccaatg ccaactgacc aactggccat attcggagca atttgaagcc aatttccatc
     1381 gcctggcgat cgctccattc ttggctatat gtttttcacc gttacccggg gccattttca
     1441 aagactcgtc ggcaagataa gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat
     1501 gctgaggaat ttcgcgatga cgtcggcgag tattttgaag aatgagaata atttgtattt
     1561 atacgaaaat cagttagtgg aattttctac aaaaacatgt tatctataga taattttgtt
     1621 gcaaaatatg ttgactatga caaagaactg gaattaagtg gcatattttg cattgctttc
     1681 cgataatagt tgtattttta taaagtaaag cgtttgtaaa ataaatacct ttaatgtatt
     1741 ctcattttct tatgtattta taatggcaat gatgatactg atgatatttt aagatgatgc
     1801 cagaccaaaa ggcttgaatt tctgcgtctt ttgccgaacg cagtgcatgt gcaattgttg
     1861 ttttttggaa tattcaattt tcggactgtc cgctttgatt tcagtttctt ggcttattca
     1921 aaaagcaaag taaagccaaa aaagcgagat ggcaatacca aatgcggcaa aacggtagtg
     1981 gaaggaaagg ggtgcggggc agcggaagga agggtggggc ggggcgtggc ggggtctgtg
     2041 gctgggcgcg acgtcaccga cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat
     2101 tattcgtgtc tcgccactcg ccggttgttt ttttcttttt atgctgcgct ctctctagcg
     2161 ccatctcgct tacgcatgct caacgcaccg catgttgccg tttcctttta tgcgtcattt
     2221 tggctcgaaa taggcaatta tttaaacaaa gattagtcaa cgaaaacgct aaaataaata
     2281 agtctacaat atggttactt attgccatgt gtgtgcagcc aacgatagca acaaaagcaa
     2341 caacacaggt ggctttccct ctttcacttt ttgtttgcaa gccgcgtgcg agcaagacgg
     2401 cacgaccggc aaacgcaatt acgctgacaa agagcagacg aagttttggc gaaaaacatc
     2461 aaggcgcctg atacgaatgc atttgcaata acaattgcga tatttaatat tgtttatgaa
     2521 gctgtttgac ttcaaaacac acaaaaaaaa aaataaaaca aattatttga aagagaatta
     2581 ggaatcggac gcttatcgtt agggtaacaa caagaaatgc ttactgagtc acagcctctg
     2641 gaaaactgcc gcaagccaga gagagagaga aaaagaggga gagcagctta gaccgcatgt
     2701 gcttgtgtgt gaggcgtctc tctcttcgtc tctgttgcgc aaacgcatag actgcactga
     2761 aaaaatcgat tacctatttt ttatgaatga atatttgcac tattactatt caaaactatt
     2821 aagatagcaa tcacattcaa tagccaaata ctataccacc tgagcgatgc aacgaaatga
     2881 tcaatttgag caaaaatgct gcatatttag gacggcatca ttatagaaat gcttcttgct
     2941 gtgtactttt ctctcgtctg gcagctgttt cgccgttatt gttaaaaccg gcttaagtta
     3001 ggtgtgtttt ctacgactag tgaatgccct actagaagat gtgtgttgca caaaatgtcc
     3061 ctggaataac caatttgaag tgcagatagc agtaaacgta agctaatatg aatattattt
     3121 aactgtaatg ttttaatatc gctggacatt actaataaac ccactataaa cacatgtaca
     3181 tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa tgtgtaaaag caccgtgacc
     3241 atcacagcat aaagataacc agctgaagta tcgaatatga gtaaccccca aattgaatca
     3301 catgccgcaa ctgataggac ccatggaagt acactcttca tggcgatata caagacacac
     3361 acaagcacga acacccagtt gcggaggaaa ttctccgtaa atgaaaaccc aatcggcgaa
     3421 caattcatac ccatatatgg taaaagtttt gaacgcgact tgagagcgga gagcattgcg
     3481 gctgataagg ttttagcgct aagcgggctt tataaaacgg gctgcgggac cagttttcat
     3541 atcactaccg tttgagttct tgtgctgtgt ggatactcct cccgacacaa agccgctcca
     3601 tcagccagca gtcgtctaat ccagagaccc cggatcccca tggtgcgctc ctccaagaac
     3661 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag
     3721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg
     3781 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
     3841 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
     3901 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3961 gtgacccaag actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc
     4021 gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4081 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggccctgaag
     4141 ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc
     4201 gtgcagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag
     4261 gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag
     4321 tctagatgta cgaacacgca tttatcatat aataaatcga taactttggc aaaaaaaaaa
     4381 aaaaaaaaaa aaaaanctcg ngggggggcc cgctagagat cttccatacc taccagttct
     4441 gcgcctgcag caatggcaac aacgttgccc ggatcgaggt cgactaaggc caaagagtct
     4501 aatttttgtt catcaatggg ttataacata tgggttatat tataagtttg ttttaagttt
     4561 ttgagactga taagaatgtt tcgatcgaat attccataga acaacaatag tattacctaa
     4621 ttaccaagtc ttaatttagc aaaaatgtta ttgcttatag aaaaaataaa atnatttatt
     4681 tgaaatttaa agtcaacttg tcatttaatg tcttgtagac ttttgaaagt cttacgatac
     4741 aattagtatc taatatacat gggttcattc tacattctat attagtgatg atttctttag
     4801 ctagtaatac attttaatta tattcggctt tgatgatttt ctgatttttt ccgaacggat
     4861 tttcgtagac cctttcgatc tcataatggc tcattttatt gcgatggacg gtcaggagag
     4921 ctccactttt gaatttctgt tcgcagacac cgcatttgta gcacatagcc gggacatccg
     4981 gtttggggag attttccagt ctctgttgca attggttttc gggaatgcgt tgcaggcgca
     5041 tacgctctat atcctccgaa cggcgctggt tgaccctagc atttacataa ggatcagcag
     5101 caaaatttgc ctctacttca ttgcccggaa tcacagcaat cagatgtccc tttcggttac
     5161 gatggatatt caggtgcgaa ccgcacacaa agctctcgcc gcacactcca cactgatatg
     5221 gtcgctcgcc cgtgtggcgg ccgcctgcag ctggcgccat cgatacgcgt acgtcgcgac
     5281 cgcggacatg tacagagctc gagaagtact agtggccacg tgggccgtgc accttaagct
     5341 tggcgcgcct aggccggccg ctagcagatc tcatcgcttt gcagaagagc agagaggata
     5401 tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag
     5461 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5521 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5581 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5641 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     5701 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
     5761 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
     5821 aaccacattg tggccaagct gtgctcgaca tgcccgccgt gaccgtcgag aacccgctga
     5881 cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca
     5941 ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca
     6001 actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc
     6061 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
     6121 tcgtcgagga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6361 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6421 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6481 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6601 ggtggcgaaa cccgacagga ctataaagat accagcggtt tccccctgga agctccctcg
     6661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6721 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6961 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8281 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8341 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8401 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     8461 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     8521 cagagcagat tgtactgaga gtgcaccata tgggtattca cgacagcagg ctgaataata
     8581 aaaaaattag aaactattat ttaaccctag aaagataatc atattgtgac gtacgttaaa
     8641 gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta
     8701 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
     8761 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
     8821 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
     8881 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
     8941 aacaaattat acttattagt cagtccagaa acaactttgg cacctgcagg tcatcacaga
     9001 acacatttgg tctagcgtgt ccactccgcc tttagtttga ttataataca taaccatttg
     9061 cggtttaccg gtactttcgt tgatagaagc atcctcatca caagatgata ataagtatac
     9121 catcttagct ggcttcggtt tatatgagac gagagtaagg ggtccgtcaa aacaaaacat
     9181 cgatgttccc actggcctgg agcgactgtt tttcagtact tccggtatct cgcgtttgtt
     9241 tgatcgcacg gttcccacaa tggttaattc gagctcgccc ggggatctaa ttcaattaga
     9301 gactaattca attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga
     9361 ccgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca
     9421 aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa
     9481 acaatcgggg taccgctaga gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     9541 atgcc
//
