pHM1 vector (V005533)

LOCUS       V005533                12702 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005533
VERSION     V005533
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang
            G-L., White FF, Yin Z.
  TITLE     R gene expression induced by a type-III effector triggers disease
            resistance in rice
  JOURNAL   Nature 435 (7045), 1122-1125 (2005)
   PUBMED   15973413
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Yin Z, Tian D.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2006) Molecular Plant Pathology, Temasek Life
            Sciences Laboratory, 1 Research Link, National University of
            Singapore, Singapore 117604, Singapore
REFERENCE   3  (bases 1 to 12702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
            date: "2005"; volume: "435"; issue: "7045"; pages: "1122-1125"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2006) Molecular Plant Pathology, Temasek Life Sciences
            Laboratory, 1 Research Link, National University of Singapore,
            Singapore 117604, Singapore"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     144..160
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    161..217
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(230..246)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(254..270)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(278..308)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(323..344)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    453..498
                     /label="orf84"
                     /note="orf84"
     CDS             complement(495..779)
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label="parB"
                     /note="partition protein; repressor and autoregulator of
                     partition operon"
                     /protein_id="ABL59975.1"
                     /translation="MNDFKLDLGSLKRTAKPASEAAIAKADAVGEARGFVDRSPRGKPG
                     RKKSERTGQVHAKVLPHVSEEIAEEAMRRGVVQGVIIEEAWALYLEAKG"
     gene            complement(495..779)
                     /gene="parB"
                     /label="parB"
     CDS             complement(776..1444)
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label="parA"
                     /note="partition protein; ATPase"
                     /protein_id="ABL59976.1"
                     /translation="MPVVVVASSKGGAGKSTTAVVLGTELAHKGVPVTMLDCDPNRSLT
                     IWANAGEVPENITALSDVTESSIVKTIKQHDVDGAVVIVDLEGVASRMVSRAISQADLV
                     LIPMRPKALDATIGAQSLQLIAEEEEAIDRKIAHAVVFTMVSPAIRSHEYTGIKASLIE
                     NGVEIIEPPLVERTAYSALFQFGGNLHSMKSKQGNMAAAIENAEAFAMAIFKKLTEALR
                     "
     gene            complement(776..1444)
                     /gene="parA"
                     /label="parA"
     rep_origin      1715..1821
                     /label="oriV"
                     /note="oriV"
     misc_feature    1997..2211
                     /label="cos region from phage lambda"
                     /note="cos region from phage lambda"
     misc_feature    2074..2086
                     /label="cos site"
                     /note="cos site"
     CDS             complement(2998..3282)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label="traJ"
                     /note="oriT-recognizing protein"
                     /protein_id="ABL59978.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATI"
     gene            complement(2998..3282)
                     /gene="traJ"
                     /label="traJ"
     oriT            complement(3315..3424)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3650..4189
                     /codon_start=1
                     /gene="traK"
                     /product="TraK"
                     /label="traK"
                     /note="oriT-binding protein"
                     /protein_id="ABL59977.1"
                     /translation="MPKSYTDELAEWVESRAAKKRRRDEAAVAFLAVRADVEAALASGY
                     ALVTIWEHMRETGKVKFSYETFRSHARRHIKAKPADVPAPQAKAAEPAPAPKTPEPRRP
                     KQGGKAEKPAPAAAPTGFTFNPTPDKKDLSHLRKAEREDGRPGPRPRAPGARGRRADPR
                     HGPRADAAAGALRSAS"
     gene            3650..4189
                     /gene="traK"
                     /label="traK"
     CDS             4500..5054
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label="aph"
                     /note="Km resistance protein"
                     /protein_id="ABL59979.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLIHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKL"
     gene            4500..5054
                     /gene="aph"
                     /label="aph"
     CDS             complement(5309..6097)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             6246..7256
                     /gene="int"
                     /label="Integrase/recombinase"
                     /note="Integrase/recombinase from Escherichia coli.
                     Accession#: P62590"
     rep_origin      complement(10156..10591)
                     /direction=LEFT
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             complement(10631..11599)
                     /label="RepA"
                     /note="replication protein of plasmid pSa"