LOCUS       V005533                12702 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005533
VERSION     V005533
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang
            G-L., White FF, Yin Z.
  TITLE     R gene expression induced by a type-III effector triggers disease
            resistance in rice
  JOURNAL   Nature 435 (7045), 1122-1125 (2005)
   PUBMED   15973413
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Yin Z, Tian D.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2006) Molecular Plant Pathology, Temasek Life
            Sciences Laboratory, 1 Research Link, National University of
            Singapore, Singapore 117604, Singapore
REFERENCE   3  (bases 1 to 12702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
            date: "2005"; volume: "435"; issue: "7045"; pages: "1122-1125"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2006) Molecular Plant Pathology, Temasek Life Sciences
            Laboratory, 1 Research Link, National University of Singapore,
            Singapore 117604, Singapore"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     144..160
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    161..217
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(230..246)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(254..270)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(278..308)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(323..344)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    453..498
                     /label="orf84"
                     /note="orf84"
     CDS             complement(495..779)
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label="parB"
                     /note="partition protein; repressor and autoregulator of
                     partition operon"
                     /protein_id="ABL59975.1"
                     /translation="MNDFKLDLGSLKRTAKPASEAAIAKADAVGEARGFVDRSPRGKPG
                     RKKSERTGQVHAKVLPHVSEEIAEEAMRRGVVQGVIIEEAWALYLEAKG"
     gene            complement(495..779)
                     /gene="parB"
                     /label="parB"
     CDS             complement(776..1444)
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label="parA"
                     /note="partition protein; ATPase"
                     /protein_id="ABL59976.1"
                     /translation="MPVVVVASSKGGAGKSTTAVVLGTELAHKGVPVTMLDCDPNRSLT
                     IWANAGEVPENITALSDVTESSIVKTIKQHDVDGAVVIVDLEGVASRMVSRAISQADLV
                     LIPMRPKALDATIGAQSLQLIAEEEEAIDRKIAHAVVFTMVSPAIRSHEYTGIKASLIE
                     NGVEIIEPPLVERTAYSALFQFGGNLHSMKSKQGNMAAAIENAEAFAMAIFKKLTEALR
                     "
     gene            complement(776..1444)
                     /gene="parA"
                     /label="parA"
     rep_origin      1715..1821
                     /label="oriV"
                     /note="oriV"
     misc_feature    1997..2211
                     /label="cos region from phage lambda"
                     /note="cos region from phage lambda"
     misc_feature    2074..2086
                     /label="cos site"
                     /note="cos site"
     CDS             complement(2998..3282)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label="traJ"
                     /note="oriT-recognizing protein"
                     /protein_id="ABL59978.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATI"
     gene            complement(2998..3282)
                     /gene="traJ"
                     /label="traJ"
     oriT            complement(3315..3424)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3650..4189
                     /codon_start=1
                     /gene="traK"
                     /product="TraK"
                     /label="traK"
                     /note="oriT-binding protein"
                     /protein_id="ABL59977.1"
                     /translation="MPKSYTDELAEWVESRAAKKRRRDEAAVAFLAVRADVEAALASGY
                     ALVTIWEHMRETGKVKFSYETFRSHARRHIKAKPADVPAPQAKAAEPAPAPKTPEPRRP
                     KQGGKAEKPAPAAAPTGFTFNPTPDKKDLSHLRKAEREDGRPGPRPRAPGARGRRADPR
                     HGPRADAAAGALRSAS"
     gene            3650..4189
                     /gene="traK"
                     /label="traK"
     CDS             4500..5054
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label="aph"
                     /note="Km resistance protein"
                     /protein_id="ABL59979.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLIHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKL"
     gene            4500..5054
                     /gene="aph"
                     /label="aph"
     CDS             complement(5309..6097)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             6246..7256
                     /gene="int"
                     /label="Integrase/recombinase"
                     /note="Integrase/recombinase from Escherichia coli.
                     Accession#: P62590"
     rep_origin      complement(10156..10591)
                     /direction=LEFT
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             complement(10631..11599)
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
ORIGIN
        1 gatccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
       61 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      121 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
      181 gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
      241 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      301 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      361 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      421 cggggagagg cggtttgcgt attgggatcc cgggccctcg acggaatgtt caatcctgta
      481 gcgcatttgc tccgtcatcc tttggcctcc aaatacagtg cccatgcttc ctctataatg
      541 acgccttgca cgacgccacg ccgcatagct tcctctgcga tttcttcgga aacgtgcggc
      601 agcactttgg cgtgcacctg gccggtgcgc tcgctcttct tgcgaccggg ctttccgcgc
      661 ggcgaccgat caacgaaccc cctcgcctcc cctactgcgt cagccttggc gatggccgct
      721 tcgctggccg gcttcgcggt ccgtttcagc gaccctagat ctagtttgaa atcattcatc
      781 gcagtgcctc cgttagcttc ttaaatattg ccatggcgaa ggcctcggcg ttttcgattg
      841 ccgccgccat attgccttgc ttcgatttca ttgaatggag attgccgccg aactgaaaaa
      901 gcgcggaata tgccgtccgc tctacaagtg gcggttcgat gatttctact ccgttttcga
      961 tgagtgatgc cttgatgccg gtgtattcat gtgaacggat cgcggggcta accatggtga
     1021 acacgaccgc atgagctatt ttccggtcta ttgcttcttc ttcttctgca atcagctgta
     1081 gcgactgcgc gcctatggtt gcgtccagtg ccttggggcg cattggaatt agcaccaagt
     1141 ctgcttgaga tattgctcta gagaccatgc gcgacgctac accttcaagg tcaacaatga
     1201 ccacggctcc atcaacgtca tgctgcttga tcgtcttgac gatgctggat tcggttacgt
     1261 cgcttagagc tgtgatattc tccgggactt cccccgcgtt tgcccaaata gtcaatgacc
     1321 ggttagggtc gcaatccaac atggtgacgg ggacaccctt atgcgcaagc tcggttccca
     1381 gcaccacggc ggtggtggat ttccctgccc cgccctttga tgaagccact acgacaacag
     1441 gcatatgtca ttctccggtt aaatcgataa tctggtttat tcaaataccc ggtaaaagtt
     1501 gatatcggat taataattaa atccggtaaa tgaattgacc ggatttaaac acacacccgg
     1561 ttttgattgc aaccgggtat ttacacaaat ccgataatta caattaccgg ataagcgaaa
     1621 ttacccggta tttcaatgaa ccgggtatcg catcaaatcg gatatgccga ctggctaaag
     1681 taaagaagca gttaattttc aatgcgggga attggtgtct cgcacacggc ttcgacggcg
     1741 tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc
     1801 cggtgagcgt cggaaaggcg ctggaagccc cgtagcgacg cggagagggg cgagacaagc
     1861 caagggcgca ggctcgatgc gcagcacgac atagccggtt ctcgcaagga cgagaatttc
     1921 cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc gagccattcg cgagagcctt
     1981 gagtccacgc tagatccctc cacgcacgtt gtgatatgta gatgataatc attatcactt
     2041 tacgggtcct ttccggtgat ccgacaggtt acggggcggc gacctcgcgg gttttcgcta
     2101 tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac ttaatgtttt
     2161 tatttaaaat accctctgaa aagaaaggaa acgacaggtg ctgaaagcga gctttttggg
     2221 atctcgtcta ccgtgtaccc actctcacgg ccgacgaaac gcttaaagga acaatagcca
     2281 tacgttcgct tctcacgctg caacgttatc aggcatggcg gcagctcgtt gctgaatagc
     2341 ttctcattga aaaaatcgta ggctctctga agttcatcgt aggtttcggg tgtaggcaaa
     2401 ttcactgcta acctcacata tatgtattgt gcaatacaca taatttagat gatcggtcga
     2461 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     2521 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     2581 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     2641 caaggcccga tctgctcggc cattccgacg tctctacgac gatgatttac acgcatgtgc
     2701 tgaaagttgg cggtgccgga gtgcgctcac cgcttgatgc gctgccgccc ctcactagtg
     2761 agaggtaggg cagcgcaagt caatcctggc ggattcacta cccctgcgcg aaggccatcg
     2821 gtgccgcatc gaacggccgg ttgcggaaag tcctccctgc gtccgctgat ggccggcagc
     2881 agcccgtcgt tgcctgatgg atcagcaagc agaattttca tttgcttaat atcatcttcc
     2941 gaaagtgacg gttttcttcc ccctacacgg ccccttgcgc gagcagctgc gagtccagat
     3001 cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc
     3061 gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac
     3121 gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc
     3181 ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat
     3241 aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
     3301 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
     3361 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
     3421 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
     3481 tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca
     3541 gggttatgca gcggaaaagc gctgcttccc tgctgttttg tggaatatct accgactgga
     3601 aacaggcaaa tgcaggaaat tactgaactg aggggacagg cgagagacga tgccaaagag
     3661 ctacaccgac gagctggccg agtgggttga atcccgcgcg gccaagaagc gccggcgtga
     3721 tgaggctgcg gttgcgttcc tggcggtgag ggcggatgtc gaggcggcgt tagcgtccgg
     3781 ctatgcgctc gtcaccattt gggagcacat gcgggaaacg gggaaggtca agttctccta
     3841 cgagacgttc cgctcgcacg ccaggcggca catcaaggcc aagcccgccg atgtgcccgc
     3901 accgcaggcc aaggctgcgg aacccgcgcc ggcacccaag acgccggagc cacggcggcc
     3961 gaagcagggg ggcaaggctg aaaagccggc ccccgctgcg gccccgaccg gcttcacctt
     4021 caacccaaca ccggacaaaa aggatctatc tcatctgcgc aaggcagaac gtgaagacgg
     4081 ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgcgcca
     4141 cggcccacga gcggacgccg cagcaggagc gctgaggtct gcctcgtgaa gaaggtgttg
     4201 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     4261 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     4321 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     4381 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     4441 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     4501 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
     4561 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     4621 atcgattgta tgggaagccc gatgcgccag agttgtttct gatacatggc aaaggtagcg
     4681 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     4741 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     4801 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     4861 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     4921 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     4981 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     5041 aaatgcataa gctagcttag taaagccctc gctagatttt aatgcggatg ttgcgattac
     5101 ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc tcccaatttg
     5161 tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc
     5221 tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc
     5281 gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca
     5341 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     5401 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     5461 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     5521 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     5581 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     5641 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     5701 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     5761 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     5821 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     5881 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     5941 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     6001 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     6061 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
     6121 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     6181 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     6241 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     6301 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     6361 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     6421 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     6481 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     6541 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     6601 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc
     6661 ttctgtatgg aacgggcatg cggatcagtg agggtttgca actgcgggtc aaggatctgg
     6721 atttcgatca cggcacgatc atcgtgcggg agggcaaggg ctccaaggat cgggccttga
     6781 tgttacccga gagcttggca cccagcctgc gcgagcagct gtcgcgtgca cgggcatggt
     6841 ggctgaagga ccaggccgag ggccgcagcg gcgttgcgct tcccgacgcc cttgagcgga
     6901 agtatccgcg cgccgggcat tcctggccgt ggttctgggt ttttgcgcag cacacgcatt
     6961 cgaccgatcc acggagcggt gtcgtgcgtc gccatcacat gtatgaccag acctttcagc
     7021 gcgccttcaa acgtgccgta gaacaagcag gcatcacgaa gcccgccaca ccgcacaccc
     7081 tccgccactc gttcgcgacg gccttgctcc gcagcggtta cgacattcga accgtgcagg
     7141 atctgctcgg ccattccgac gtctctacga cgatgattta cacgcatgtg ctgaaagttg
     7201 gcggtgccgg agtgcgctca ccgcttgatg cgctgccgcc cctcactagt gagaggtagg
     7261 gcagcgcaag tcaatcctgg cggattcact acccctgcgc gaaggccatc ggtgccgcat
     7321 cgaacggccg gttgcggaaa gtcctccctg cgtccgctga tggccggcag cagcccgtcg
     7381 ttgcctgatg gatcttcctg cattgctcac gcagctttga aacctgaatc ggcgctgcta
     7441 cgcccacgtg ttctgccaac ataacggcgg atgcttcatc acgggaaagc accggaaaca
     7501 cggcgacctc atgggccaaa agccacattg aacgggtcat gccatcagtg aaacacaccg
     7561 cgaaatggcc cagaatcatg gtcgggctta cgtcagcaag cggaacgggg ctgtcgatgc
     7621 ccacggcaaa atgcgcggcg ctcgacggat atttccggtc tttaggcatg tcctgcttca
     7681 acgggcaggc ttgagaatcc tcgacgccta acgccaacca cgctcgatag aaagccaagc
     7741 cattcacata cacaatgaaa tcggactgtg cggcatttga agctgtcata aaggcgtcag
     7801 gatggttcgg cacgggcagc cgccaaacaa cctcattcca agcattcggc acgatttgag
     7861 gcaattcgag gggcaacccg ctcgactgcg caagctcttt atcaagccgc cgaatttcca
     7921 gccaagcgcg acaccgccgc cgtagcaaca tcagcatcgt tagcgcccat gctctcggcc
     7981 tgcgccggtt ttttccatct gccgctgtcg ttgatgttgc gcggcctgtg ggttgcggac
     8041 gattccggcg cgaccgtgcg gataactgat ttccgcatgg tcgttcatct tcactttctc
     8101 cgcgcccgaa acagcggcgc gttcatgcag aatgatcgtg cctgggcggc tatcgtcggc
     8161 ctgaatgaca tagctattgg tgcgaccgat cacacggccc cggtaagttt cgcccggttc
     8221 tgcgtggcgg tgaaattcat ctgcgcgggc tttctccctg cctactgcct cgcgcacctg
     8281 ttgcgcctcg atgatggccc tttctacgtc gcgctcaagc cggttggcgt tggtgctctt
     8341 agtggcgtct ggcgtggttt ctagctcttt gcgggtctct tgtagtccgc tcttgtagtt
     8401 ctcaagcaag ggttcattgc ctttggcttc tttcggaatt tcggccaatt ggcggcctgc
     8461 ctcgaaagcc agcaggcgca tggtttccgc ttcattctgg cgcgactgct cttgcagctt
     8521 ccggcgttcg tctttctcct gctcgcggcg cgtgtcggtc atggtctttc ctccatcgtg
     8581 tcaagctcgc ttcgcgtgcc atagtgctcg cgccgttcaa cggcgttgcc gtcctccact
     8641 acgttgcggc ccttcgggtg actcggcgct gtgcgctatg gcgatctgga agtgtcggga
     8701 cgaggggcgt cccggcggta aattcgacgt gcctgcggcg cgtcgaacaa ggggcgtgcc
     8761 cggtgtcagt tcgtggcatt tttcgaggcg cgacgccatt tccaaggctc ctgagcattc
     8821 gggtctgacc aaaggccgag ccgttggcgg cgggcctctt tttcatactc gtacatctgc
     8881 gcgtcggttg gtttgccgta ataacggtaa gcccaggcca tgccttcttg aaccatgatc
     8941 gcattgatgt tggtgagttg tgtttggccg ccggggtatt gcaacggcgc gtaaacgacc
     9001 ccaagagtgc ggccataccg atcaacctct ttttcggtca cttgaacctc ttggcgaaag
     9061 gtcaagtcgg cgagccgttg gcgagcacgg gagccgaagg cttggccgct ttccggtgcg
     9121 tcaatatcgg ccaatctcac gcggatggtc tgacggttca ccaaaacgtc gatagtgtca
     9181 ccgtcaagga ttcggacgac ttcaccccgg aagtcggccc aagcgggcac actgacgatt
     9241 aggacgacag cggccgcgac cgcgcgaagg gcggcaaggg cgcttttcat tgtttgcctc
     9301 ctgttttcaa gacggctgtg agattggcga cctgctcttt gagggcttcc acctgacctt
     9361 gcagactggc ggcgcgctcg atggcctcct tggcctgttt gcgggcctcg atagcctcgt
     9421 tatcgcgttg ggtgagcttt tccatgcagc ggtttagttc ttcgccgctg cggcgtttca
     9481 cttcggcaag ctggtcggcc agcttgtcgc gctcgcgttc cataggttcg agctgattca
     9541 ctcgttcgcg gagctggtcg ttttcgcggg tgaaggtgtc ggctagttcg attgcttcgg
     9601 caagctgctg gctgatggcc gctttgtcgg cctcgatctg tttccgatct tcgtcaaacc
     9661 gggcgttggc gtgcgccagg gcgatagccc atagcgcatt gccaagctcg gcaagatgct
     9721 cgttgactgc aaccggcaat gggtctgatg agggcagggt ggcggtcttg cggtttttcc
     9781 attcagccat tgcatcggaa atggttgtga agctaccgct tccgagtttc ttgcgcacgg
     9841 cggccaaagt gggccggatg ccttcggcgt ccagttcgtc ggctgctcgc caaatgtctt
     9901 gtttagtgat tgccattctt gcgggcctct gtactgtagt atgttgtatg atactacata
     9961 ctacaacaat ttaacagagc catcttggaa tctggtgtct ctgcgcctat aattctggaa
    10021 cagctacttt ccgaacgact cctgcgttga tcggaaatcc agaagcccga gaggttgccg
    10081 cctttcgggc tttttctttt tcaaaaaaaa aaatttataa aacgatctgt tgcggccgcc
    10141 gggttgtggg caaaggcgct cgacggtggg caaccgcttg cggttgtcca cgggcggagc
    10201 cggtgcgcgt agcgcattgt ccacaagcca agggcgacca ataattgata tatatattca
    10261 taattgaaaa gctaattgaa catactactt gctgtaacta cttgccggag cgaggggtgt
    10321 ttgcaagctg ttgatctgaa agggctatta gcgttctcac gtgccttttt gattagcgat
    10381 ttcacgtgac cttattagcg atttcacgta ctccgattag cgatttcacg taccctgatt
    10441 agcgatttca cgtggatagt ttttggagcg ggccggaaag ccccgtgaat caaggctttg
    10501 cggggcatta gcggtttcac gtggataact accctctatc cacaggcttc cggggataaa
    10561 aaagcccgct cgacggcggg ctgttggatg ggaaggcttg accaagccaa gcgtagcgtt
    10621 ggcctggtca agtcggaggg gggccgatgc gagcgccctt gccgggtgcg cgggtgacat
    10681 gcaggcgtgt ggatttgatg cgcaggcatt cgccgtcatc ttcgatgcag tcgcttgcct
    10741 cgggatagac aatcaacact tcgcgtaggc gctttttgaa gttgtatttg aagctggcga
    10801 gtgctgcccg ctctgcccgc tctcgggcct tatcgtccag ttcgggcgag ttgcgtgcgc
    10861 ggctgccata ggatgagccg aattgcgctt gcagggcgac ccaagggatt tgcacgaagg
    10921 ggcggccctt ggcccgcaac aggaacacgc gataggtcag ccacgtgtaa atgtccatcg
    10981 caagcggaga ctgccgcaag gcatgcaggt agtcgattcg gataggaacc ggtgagcggg
    11041 tgacttcctc gaagaaatcg cctgtgaggg tgagggtgct atcccatagc gcccgatctt
    11101 ctggccgctt gggattccag aatagaaaag cgcgcttggc aatgacgacg ttctcaatgc
    11161 cgaagtcatt gccttgctcg ccggcaagcg aaatcatgga tgaaaacagg cgttgcgcct
    11221 gattgcgaag ggtggccgtg taacggccat cggtgtgcat tccgagcctt tgtagaaatt
    11281 ccgattgcga ccggccaagg ttcaacacgg ggtctttcgt tcgcacggcc tcggtgcata
    11341 tccaagcaag caaggtgcgc ggcatagaac cgtagggcag gccgatgctc ggcttgccca
    11401 tgatcgacaa ggtgacgatg ccattggtgc gctcaaagta gctggtcttg gggtcggtgt
    11461 ggggcatggt cgcttgcaca aggcaacggg ccatgtagcc gactaagcca gcttcgcggg
    11521 catcctccat ttcgagcgcg aggctcgtct tgatgatctc gttgatacga tggccggggg
    11581 ctttgttgtt cttaggcatg ttgttccctc cccggcatgg tgatggttgg tctagtgttt
    11641 gtgggtttga tgttccggcg tttgatgaac aggcgcaagg tgtgagggct gacgcctaac
    11701 aactcggctg cgcgactttg cggcaagcca aggttcacgt atgcctgtac ttcatcaata
    11761 cggctgtcca gcttcaaggc gctcgatttg ctgcccttgg gtcgcccgag cgtcttgccg
    11821 cgctctctgg cgacttgtag cgcctcggtg gtacgtgcct gaatgaaatg ccgctcgatc
    11881 tgtgcagcca agccaagcac ggttgccatg atgtcgcttt gtaggctgcc gtccatgatg
    11941 atcttctgtt tggtcacatg gacgattagg ccgcgctcgc tcgccgcttt gagaatttcc
    12001 aaggcggcga gggcggaacc ggcaatgcgc gtaatctccg gcgtcagtag cacgtcgcca
    12061 cgctcggcct tttcgatgat tgctccgagc ttgcgcttgc gccagtcctt tgctctgctg
    12121 gcaatttctt cctcgatctg tagcggcgcg aagcctttgg cgttcgcgta ttcgagcaaa
    12181 ccgtattttt ggttttccgg gtcttggccg tcacgcgaaa cccggagata ggcatagtat
    12241 tttggcattt gcagggaaaa cgtcagattc ggttaaacat gcctcattct agcgcagatt
    12301 aaataggaat taaataccct gttgcggtat agataaaacg ttggtttgtt ctgccctatg
    12361 agcgtacaaa aaaggccggg tgagtggccc ggccttcgtt taggtgctga ataggattgg
    12421 ttctggtgcc agcctcatga gaagcgcgtc ataaaaccac atgagggccg acgcaccaag
    12481 gccgacgcct gcgaccgata gcatgatgtg ggtcttattg gccgagtcca gcccaagcca
    12541 catgatcggt agggtgatga gactggcgaa cgaagccaag ccgagaagaa agcgcaccgg
    12601 gccgcgcagc caccagagaa tgaggaacac cagatgtcgt tttcagaaga cggctgcact
    12661 gaacgtcaga agccgactgc actatagcag cggaggggtt gg
//
