pHIGH100 vector (V005559)

LOCUS       V005559                 5313 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005559
VERSION     V005559
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden
            PD.
  TITLE     Creation and characterisation of a high-copy-number version of the
            pAL5000 mycobacterial replicon
  JOURNAL   Tuberculosis (Edinb) 87 (6), 481-488 (2007)
   PUBMED   17888739
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5313)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5313)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6";
            pages: "481-488"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease
            and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      250..838
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1126..1147
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1162..1192
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1200..1216
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1224..1240
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1328..1344)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1374..3958
                     /note="mycobacterial origin; OriM"
     CDS             complement(4199..5194)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"