LOCUS       V005559                 5313 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005559
VERSION     V005559
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden
            PD.
  TITLE     Creation and characterisation of a high-copy-number version of the
            pAL5000 mycobacterial replicon
  JOURNAL   Tuberculosis (Edinb) 87 (6), 481-488 (2007)
   PUBMED   17888739
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5313)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5313)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6";
            pages: "481-488"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease
            and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      250..838
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1126..1147
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1162..1192
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1200..1216
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1224..1240
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1328..1344)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1374..3958
                     /note="mycobacterial origin; OriM"
     CDS             complement(4199..5194)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
ORIGIN
        1 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
       61 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      361 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
      421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      901 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      961 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1081 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1141 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1201 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacc agatctggct
     1261 cgagctccac cgcggtggcg gccgctctag gatcccccgg gctgcaggaa ttcgatatca
     1321 agcttatcga taccgtcgac ctcgaggggg ggcccggtac gtacccgggc atcatcgagc
     1381 cgagaacgtt atcgaagttg gtcatgtgta atcccctcgt ttgaactttg gattaagcgt
     1441 agatacaccc ttggacaagc cagttggatt cggagacaag caaattcagc cttaaaaagg
     1501 gcgaggcctg cggtggtgga acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac
     1561 cagcccgcga acggctggca gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg
     1621 atgtcctgca cttcgagcca agcctcgaac acctgctggt gtgcacgact cacccggttg
     1681 ttgacaccgc gcgcggccgt gcgggctcgg tggggcggct gtgtcgccct tgccagcgtg
     1741 agtagcgcgt acctcacctc gcccaacagg tcgcacacag ccgattcgta cgccataaag
     1801 ccaggtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca
     1861 ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc
     1921 ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca ggtggttggg
     1981 ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg ggggagtgtg
     2041 cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg gaccatacac
     2101 cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc
     2161 tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc taccgccaaa
     2221 gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc
     2281 tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc gcccgggggt
     2341 cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca cacgcagtgt
     2401 gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca
     2461 tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc
     2521 tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac tcagatctct
     2581 acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc
     2641 agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca ctgttcgatt
     2701 ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctgccg acccggaacg
     2761 tggacggact cggccgcgcg atctatgccg agtgccacgc gcgaaacgcc gaattcccgt
     2821 gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc gccaacagca
     2881 tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg
     2941 ccacactcag tgcgcgccag tcggccatct cgcggaaggg agcagcgcgc acggcggcga
     3001 gcacagttgc gcggcgcgca aagtccgcgt cagccatgga ggcattgcta tgagcgacgg
     3061 ctacagcgac ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg
     3121 cgtgaccgcc gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct
     3181 cgtggcgcag gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg
     3241 cgcctatcac gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca
     3301 tctggacacc gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga
     3361 agcggctcaa aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg
     3421 agcgggtgtc gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt
     3481 cctggtagac gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg
     3541 cccgttttga ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc
     3601 atttccgctg aatatcgggg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg
     3661 cgaactccgt tgtagtgcct gtggtggcat ccgtggccac tctcgttgca cggttcgttg
     3721 tgccgttaca ggccccgttg acagctcacc gaacgtagtt aaaacatgct ggtcaaacta
     3781 ggtttaccaa cgatacgagt cagctcatct agggccagtt ctaggcgttg ttcgttgcgc
     3841 ggttcgttgc gcatgtttcg tgtggttgct agatggctcc gcaaccacac gcttcgaggt
     3901 tgagtgcttc cagcacgggc gcgatccaga agaacttcgt cgtgcgactg tcctcgttga
     3961 tccttgccga gctgggatgg aagctcggcc gaccaccctg gaggagatga tcgaggatgc
     4021 cagggccttt cacgcccgcc gctgctgagc gtccgccgcc gggcccgcac cgccgtcggc
     4081 cggcccgctc cgggctcgca gcagcgggct tcggcgcggg cccggggctc ccgagcgcgg
     4141 gcggggctcc gggcggccgc cgggggccgg gggcggcgcc gggcggcccg gggcgtcagg
     4201 cgccgggggc ggtgtccggc ggcccccaga ggaactgcgc cagttcctcc ggatcggtga
     4261 agccggagag atccagcggg gtctcctcga acacctcgaa gtcgtgcagg aaggtgaagg
     4321 cgagcagttc gcgggcgaag tcctcggtcc gcttccactg cgccccgtcg agcagcgcgg
     4381 ccaggatctc gcggtcgccc cggaaggcgt tgagatgcag ttgcaccagg ctgtagcggg
     4441 agtctcccgc atagacgtcg gtgaagtcga cgatcccggt gacctcggtc gcggccaggt
     4501 ccacgaagat gttggtcccg tgcaggtcgc cgtggacgaa ccggggttcg cggccggcca
     4561 gcagcgtgtc cacgtccggc agccagtcct ccaggcggtc cagcagccgg ggcgagaggt
     4621 agccccaccc gcggtggtcc tcgacggtcg ccgcgcggcg ttcccgcagc agttccggga
     4681 agacctcgga atggggggtg agcacggtgt tcccggtcag cggcaccctg tgcagccggc
     4741 cgagcacccg gccgagttcg cgggccaggg cgagcagcgc gttccggtcg gtcgtgccgt
     4801 ccatcgcgga ccgccaggtg gtgccggtca tccggctcat caccaggtag ggccacggcc
     4861 aggctccggt gccgggccgc agctcgccgc ggccgaggag gcggggcacc ggcaccgggg
     4921 cgtccgccag gaccgcgtac gcctccgact ccgacgcgag gctctccgga ccgcaccagt
     4981 gctcgccgaa cagcttgatc accgggtcgg gctcgccgac cagtacgggg ttggtgctct
     5041 cgccgggcac ccgcagcacc ggcggcaccg gcagcccgag ctcctccagg gctcggcggg
     5101 ccagcggctc ccagaattcc tggtcgttcc gcaggctcgc gtaggaatca tccgaatcaa
     5161 tacggtcgag aagtaacagg gattcttgtg tcacagcgga cctctattca cagggtacgg
     5221 gccggcttaa ttccgcacgg ccggtcgcga cacggcctgt ccgcaccgcg gtcaggcgtt
     5281 gacgatgacg ggctggtcgg ccacgtcggg gac
//
