pMO90 vector (V004685)

LOCUS       V004685                 4396 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004685
VERSION     V004685
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI,
            Vazquez-Torres A.
  TITLE     Inactivation of [Fe-S] metalloproteins mediates nitric
            oxide-dependent killing of Burkholderia mallei
  JOURNAL   PLoS ONE 3 (4), E1976 (2008)
   PUBMED   18398486
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M,
            Vazquez-Torres A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave.,
            Denver, CO 80045, USA
REFERENCE   3  (bases 1 to 4396)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2008"; volume: "3"; issue: "4"; pages: "E1976"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO
            80045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..992
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1071..1784
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     oriT            2111..2220
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      2519..3107
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3275..3303
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3335..4255
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"