Basic Vector Information
- Vector Name:
- pMO90
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4396 bp
- Type:
- Suicide vector
- Replication origin:
- ori
- Source/Author:
- Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A.
- Promoter:
- tac
pMO90 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMO90 vector Sequence
LOCUS 40924_31195 4396 bp DNA circular SYN 18-DEC-2018 DEFINITION Suicide vector pMO90, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4396) AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A. TITLE Inactivation of [Fe-S] metalloproteins mediates nitric oxide-dependent killing of Burkholderia mallei JOURNAL PLoS ONE 3 (4), E1976 (2008) PUBMED 18398486 REFERENCE 2 (bases 1 to 4396) AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M, Vazquez-Torres A. TITLE Direct Submission JOURNAL Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 80045, USA REFERENCE 3 (bases 1 to 4396) TITLE Direct Submission REFERENCE 4 (bases 1 to 4396) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2008"; volume: "3"; issue: "4"; pages: "E1976" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 80045, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4396 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 96..200 /label=AmpR promoter CDS 201..992 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" CDS 1071..1784 /label=GFPuv /note="GFP variant optimized for excitation by UV light" oriT 2111..2220 /label=oriT /note="incP origin of transfer" rep_origin 2519..3107 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 3275..3303 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" CDS 3335..4255 /gene="xylE" /label=xylE /note="Metapyrocatechase from Pseudomonas putida. Accession#: P06622"
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