LOCUS       V004685                 4396 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004685
VERSION     V004685
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI,
            Vazquez-Torres A.
  TITLE     Inactivation of [Fe-S] metalloproteins mediates nitric
            oxide-dependent killing of Burkholderia mallei
  JOURNAL   PLoS ONE 3 (4), E1976 (2008)
   PUBMED   18398486
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M,
            Vazquez-Torres A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave.,
            Denver, CO 80045, USA
REFERENCE   3  (bases 1 to 4396)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2008"; volume: "3"; issue: "4"; pages: "E1976"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO
            80045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..992
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1071..1784
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     oriT            2111..2220
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      2519..3107
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3275..3303
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3335..4255
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgattaat taaactagtc tgttgacaat
     1021 taatcatcga tctagtttaa tgtgtggaaa attgaaggag atagatttct atgagtaaag
     1081 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1141 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1201 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1261 tctcttatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt
     1321 tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg
     1381 ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg
     1441 agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa ctcgagtaca
     1501 actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta
     1561 acttcaaaat tcgccacaac attgaagatg ggtccgttca actagcagac cattatcaac
     1621 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctctcgacac
     1681 aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
     1741 taactgctgc tgggattaca catggcatgg atgagctcta caaataaccg cggaggaacg
     1801 caaccgcagc ctcatcacgc cggcgcttct tggccgcgcg ggattcaacc cactcggcca
     1861 gctcgtcggt gtagctcttt ggcatcgtct ctcgcctgtc ccctcagttc agtaatttcc
     1921 tgcatttgcc tgtttccagt cggtagatat tccacaaaac agcagggaag cagcgctttt
     1981 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     2041 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     2101 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     2161 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgg
     2221 ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag ccaaccagga
     2281 agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg attgaggtta
     2341 attaataact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2641 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3181 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat atcagggccc
     3241 cgctagccag atctgtcccg ggaagcttga gctgttgaca attaatcatc ggctcgtata
     3301 atgtgtggaa tatcacagaa ggagacagaa tactatgaac aaaggtgtaa tgcgaccggg
     3361 ccatgtgcag ctgcgtgtac tggacatgag caaggccctg gaacactacg tcgagttgct
     3421 gggcctgatc gagatggacc gtgacgacca gggccgtgtc tatctgaagg cttggaccga
     3481 agtggataag ttttccctgg tgctacgcga ggctgacgag ccgggcatgg attttatggg
     3541 tttcaaggtt gtggatgagg atgctctccg gcaactggag cgggatctga tggcatatgg
     3601 ctgtgccgtt gagcagctac ccgcaggtga actgaacagt tgtggccggc gcgtgcgctt
     3661 ccaggccccc tccgggcatc acttcgagtt gtatgcagac aaggaatata ctggaaagtg
     3721 gggtttgaat gacgtcaatc ccgaggcatg gccgcgcgat ctgaaaggta tggcggctgt
     3781 gcgtttcgac cacgccctca tgtatggcga cgaattgccg gcgacctatg acctgttcac
     3841 caaggtgctc ggtttctatc tggccgaaca ggtgctggac gaaaatggca cgcgcgtcgc
     3901 ccagtttctc agtctgtcga ccaaggccca cgacgtggcc ttcattcacc atccggaaaa
     3961 aggccgcctc catcatgtgt ccttccacct cgaaacctgg gaagacttgc ttcgcgccgc
     4021 cgacctgatc tccatgaccg acacatctat cgatatcggc ccaacccgcc acggcctcac
     4081 tcacggcaag accatctact tcttcgaccc gtccggtaac cgcaacgaag tgttctgcgg
     4141 gggagattac aactacccgg accacaaacc ggtgacctgg accaccgacc agctgggcaa
     4201 ggcgatcttt taccacgacc gcattctcaa cgaacgattc atgaccgtgc tgacctgagc
     4261 ggccgccctg cagcggatcc ctctagatgc atgcgacggg cttgtctgct cccggcatcc
     4321 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4381 tcaccgaaac gcgcga
//
