Basic Vector Information
- Vector Name:
- pMO79
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8021 bp
- Type:
- Replicative vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A.
pMO79 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMO79 vector Sequence
LOCUS 40924_31190 8021 bp DNA circular SYN 18-DEC-2018
DEFINITION Replicative vector pMO79, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8021)
AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI,
Vazquez-Torres A.
TITLE Inactivation of [Fe-S] metalloproteins mediates nitric
oxide-dependent killing of Burkholderia mallei
JOURNAL PLoS ONE 3 (4), E1976 (2008)
PUBMED 18398486
REFERENCE 2 (bases 1 to 8021)
AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M,
Vazquez-Torres A.
TITLE Direct Submission
JOURNAL Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave.,
Denver, CO 80045, USA
REFERENCE 3 (bases 1 to 8021)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8021)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2008"; volume: "3"; issue: "4"; pages: "E1976"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO
80045, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8021
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 96..200
/label=AmpR promoter
CDS 201..992
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 1010..1822
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
promoter 1881..1909
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 1924..2637
/codon_start=1
/label=GFPuv
/note="GFP variant optimized for excitation by UV light"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
promoter complement(3290..3392)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
misc_feature 3542..4561
/label=mobilization region
/note="mobilization region"
rep_origin 4785..5554
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS 5555..6214
/codon_start=1
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
protein_bind 6842..6863
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6878..6908
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6916..6932
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6940..6956
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 7070..7726
/codon_start=1
/label=mRFP1.1
/note="mRFP1.1 is a basic (constitutively fluorescent) red
fluorescent protein published in 2004, derived from
Discosoma sp.."
/translation="VQEETMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKG
GPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQD
SSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKD
GGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGA"
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