LOCUS       40924_31190        8021 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Replicative vector pMO79, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, 
            Vazquez-Torres A.
  TITLE     Inactivation of [Fe-S] metalloproteins mediates nitric 
            oxide-dependent killing of Burkholderia mallei
  JOURNAL   PLoS ONE 3 (4), E1976 (2008)
  PUBMED    18398486
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M, 
            Vazquez-Torres A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., 
            Denver, CO 80045, USA
REFERENCE   3  (bases 1 to 8021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2008"; volume: "3"; issue: "4"; pages: "E1976"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 
            80045, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..992
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1010..1822
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        1881..1909
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1924..2637
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(3290..3392)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3542..4561
                     /label=mobilization region
                     /note="mobilization region"
     rep_origin      4785..5554
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5555..6214
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     protein_bind    6842..6863
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6878..6908
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6916..6932
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6940..6956
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7070..7726
                     /codon_start=1
                     /label=mRFP1.1
                     /note="mRFP1.1 is a basic (constitutively fluorescent) red 
                     fluorescent protein published in 2004, derived from 
                     Discosoma sp.."
                     /translation="VQEETMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKG
                     GPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQD
                     SSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKD
                     GGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGA"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgattaat taaactagta tgagccatat
     1021 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata
     1081 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     1141 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1201 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1261 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
     1321 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1381 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1441 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1501 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1561 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1621 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1681 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1741 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1801 tttgatgctc gatgagtttt tctaattaat taatgttgac tcccttagta atttcggaat
     1861 attatgctag gttccggttc ttgacagctt atcatcgata agctataata ggaggaaacg
     1921 atgatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1981 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     2041 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     2101 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa
     2161 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
     2221 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
     2281 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
     2341 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
     2401 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atgggtccgt tcaactagca
     2461 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     2521 tacctctcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
     2581 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa
     2641 ccgcggaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga
     2701 gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt
     2761 ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc
     2821 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac
     2881 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     2941 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     3001 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     3061 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagcgaga aatcaccatg
     3121 agtgacgact gaatccggtg agaatggcaa aagctaaaaa ggccgtaata tccagctgaa
     3181 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     3241 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     3301 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     3361 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     3421 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     3481 tcacaggtat ttattcgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgcc
     3601 cgcgttcctg ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt tccgtcagca
     3661 gcttttcgcc cacggccttg atgatcgcgg cggccttggc ctgcatatcc cgattcaacg
     3721 gccccagggc gtccagaacg ggcttcaggc gctcccgaag gtctcgggcc gtctcttggg
     3781 cttgatcggc cttcttgcgc atctcacgcg ctcctgcggc ggcctgtagg gcaggctcat
     3841 acccctgccg aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct
     3901 ttgagattcc cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt
     3961 gcgggctgat ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa
     4021 tgcgcgtgtg acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag
     4081 cggccgcaaa cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg
     4141 ccgacagcct gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt
     4201 cgtcacggtg gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact
     4261 tgtgcgcctt ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg
     4321 ggctggccgt catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca
     4381 gcaactcgcg cagtcggccc atcgcttcat cggtgctgct ggccgcccag tgctcgttct
     4441 ctggcgtcct gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac
     4501 tggccgccac gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca
     4561 tgcctgcccc tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct
     4621 ccggcgggcc actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc
     4681 gcctacggcg gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc
     4741 tgcgctccct tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc
     4801 tcgcttcgct cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc
     4861 ccacgagctt gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc
     4921 ggctccaact gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa
     4981 aagcctccgg cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg
     5041 gtcaaggctc gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa
     5101 gcctatgcga gtgggggcag tcgaaggcga agcccgcccg cctgcccccc gagcctcacg
     5161 gcggcgagtg cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc
     5221 agtcgatcaa caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc
     5281 gtgggggaac cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa
     5341 atgcgaaaga cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg
     5401 caccccccgc aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt
     5461 acgcaacgca taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg
     5521 caaccgtgcg gcaccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg
     5581 cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc
     5641 gtgggccggg cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc
     5701 gcagatgggc caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg
     5761 acgttctttg cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc
     5821 cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc
     5881 gtggggccag ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga
     5941 tcacgacgac caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct
     6001 gtatccgggc gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg
     6061 cattccgggc atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg
     6121 cgggcagcag cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt
     6181 ggagccgccg acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg
     6241 taagtgcgct gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc
     6301 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     6361 gcacctcgct aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat
     6421 atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa
     6481 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     6541 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     6601 ccattcatcc gcttattatc acttattcag gcgtagcacc agatatcagg gccccgctag
     6661 ccagatctgg tcgactacgt actcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6721 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6841 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6901 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6961 attacgccaa gcttgcacgc ctgccgctcg actcttgagg acccccgggt accgagctcg
     7021 aattcctgtt gactcacttg ggattttcgg aatatcatgc cgggttccgg ttcaggagga
     7081 aacgatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     7141 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     7201 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     7261 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     7321 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     7381 ctcctccctc caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     7441 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     7501 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     7561 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     7621 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     7681 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcgcctccg gactcagatc
     7741 taagctgaac cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc
     7801 ctgacatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     7861 gacacccgcc aacacccgct gacgcggccg ccctgcagcg gatccctcta gatgcatgcg
     7921 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7981 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
