pMo130 vector (V004688)

LOCUS       V004688                 6127 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004688
VERSION     V004688
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei
  JOURNAL   Gene 430 (1-2), 123-131 (2009)
   PUBMED   19010402
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2008) Microbiology, University of Colorado Denver
            School of Medicine, 19th Ave, Aurora, CO 80010, USA
REFERENCE   3  (bases 1 to 6127)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6127)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 430
            (1-2), 123-131 (2009)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2008) Microbiology, University of Colorado Denver School of
            Medicine, 19th Ave, Aurora, CO 80010, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..992
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1176..2528
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     terminator      complement(2545..2588)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    2606..2627
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            3838..3947
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      4246..4834
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5001..5029
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             5061..5981
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"