LOCUS       V004688                 6127 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004688
VERSION     V004688
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei
  JOURNAL   Gene 430 (1-2), 123-131 (2009)
   PUBMED   19010402
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2008) Microbiology, University of Colorado Denver
            School of Medicine, 19th Ave, Aurora, CO 80010, USA
REFERENCE   3  (bases 1 to 6127)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6127)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 430
            (1-2), 123-131 (2009)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2008) Microbiology, University of Colorado Denver School of
            Medicine, 19th Ave, Aurora, CO 80010, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..992
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1176..2528
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     terminator      complement(2545..2588)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    2606..2627
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            3838..3947
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      4246..4834
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5001..5029
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             5061..5981
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgattaat taaactagtc acttcttgaa
     1021 tttgtcaaaa cccgtccata taattagcac tcgctgcaca ggaggaaacg atgacgtact
     1081 ccaaacaagc cgtttcccgt agacacaaac gtttggccct gagcgccgcc gcgctcaccg
     1141 cagccgcttg catgtcggcg cacgcgcaga gcgacggcgc aactcaagcg tttgcgaaag
     1201 aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata
     1261 tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agtttctgaa tttgattcgt
     1321 ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac
     1381 aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg
     1441 gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa
     1501 cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg
     1561 caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat
     1621 ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa
     1681 cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg
     1741 tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt
     1801 tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg
     1861 tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct
     1921 accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc
     1981 gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg
     2041 gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc
     2101 tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg
     2161 gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt
     2221 ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc
     2281 tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt
     2341 actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga
     2401 caaacagagg attctacgca gacaaacaat caacgtttgc gccgagcttc ctgctgaaca
     2461 tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag
     2521 ttaacaaata atagtagctt tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2581 cgttttaatc tgagaattcc gcaattaatg taagttagct cactcattag gcaccgggat
     2641 ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     2701 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2761 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     2821 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     2881 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg
     2941 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
     3001 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
     3061 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
     3121 cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
     3181 taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt
     3241 ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
     3301 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca
     3361 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa
     3421 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
     3481 acgagctgga cgcggatgaa caggcagaca tctgccgcgg aggaacgcaa ccgcagcctc
     3541 atcacgccgg cgcttcttgg ccgcgcggga ttcaacccac tcggccagct cgtcggtgta
     3601 gctctttggc atcgtctctc gcctgtcccc tcagttcagt aatttcctgc atttgcctgt
     3661 ttccagtcgg tagatattcc acaaaacagc agggaagcag cgcttttccg ctgcataacc
     3721 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     3781 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     3841 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     3901 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt
     3961 aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc
     4021 tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggttaatt aataactgtc
     4081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4381 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4921 tgtggataac cgtattaccg cctttgagtg agctgatatc agggccccgc tagccagatc
     4981 ttcccgggaa gcttgagctg ttgacaatta atcatcggct cgtataatgt gtggaatatc
     5041 acagaaggag acagaatact atgaacaaag gtgtaatgcg accgggccat gtgcagctgc
     5101 gtgtactgga catgagcaag gccctggaac actacgtcga gttgctgggc ctgatcgaga
     5161 tggaccgtga cgaccagggc cgtgtctatc tgaaggcttg gaccgaagtg gataagtttt
     5221 ccctggtgct acgcgaggct gacgagccgg gcatggattt tatgggtttc aaggttgtgg
     5281 atgaggatgc tctccggcaa ctggagcggg atctgatggc atatggctgt gccgttgagc
     5341 agctacccgc aggtgaactg aacagttgtg gccggcgcgt gcgcttccag gccccctccg
     5401 ggcatcactt cgagttgtat gcagacaagg aatatactgg aaagtggggt ttgaatgacg
     5461 tcaatcccga ggcatggccg cgcgatctga aaggtatggc ggctgtgcgt ttcgaccacg
     5521 ccctcatgta tggcgacgaa ttgccggcga cctatgacct gttcaccaag gtgctcggtt
     5581 tctatctggc cgaacaggtg ctggacgaaa atggcacgcg cgtcgcccag tttctcagtc
     5641 tgtcgaccaa ggcccacgac gtggccttca ttcaccatcc ggaaaaaggc cgcctccatc
     5701 atgtgtcctt ccacctcgaa acctgggaag acttgcttcg cgccgccgac ctgatctcca
     5761 tgaccgacac atctatcgat atcggcccaa cccgccacgg cctcactcac ggcaagacca
     5821 tctacttctt cgacccgtcc ggtaaccgca acgaagtgtt ctgcggggga gattacaact
     5881 acccggacca caaaccggtg acctggacca ccgaccagct gggcaaggcg atcttttacc
     5941 acgaccgcat tctcaacgaa cgattcatga ccgtgctgac ctgacctgag cggccgccct
     6001 gcagcggatc cctctagatg catgcgacgg gcttgtctgc tcccggcatc cgcttacaga
     6061 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6121 cgcgcga
//
