Basic Vector Information
- Vector Name:
- pmKATE2rrnB
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8381 bp
- Type:
- Fluorescent reporter vector
- Replication origin:
- ori
- Source/Author:
- van Gestel J, Weissing FJ, Kuipers OP, Kovacs AT.
pmKATE2rrnB vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmKATE2rrnB vector Sequence
LOCUS 40924_31035 8381 bp DNA circular SYN 18-DEC-2018
DEFINITION Fluorescent reporter vector pmKATE2rrnB, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8381)
AUTHORS van Gestel J, Weissing FJ, Kuipers OP, Kovacs AT.
TITLE Density of founder cells affects spatial pattern formation and
cooperation in Bacillus subtilis biofilms
JOURNAL ISME J 8 (10), 2069-2079 (2014)
PUBMED 24694715
REFERENCE 2 (bases 1 to 8381)
AUTHORS Kovacs AT.
TITLE Direct Submission
JOURNAL Submitted (14-JUN-2013) Terrestrial Biofilms Group, Friedrich
Schiller University of Jena, Neugasse 23, Jena, Thuringia 07743,
Germany
REFERENCE 3 (bases 1 to 8381)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8381)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ISME J";
date: "2014"; volume: "8"; issue: "10"; pages: "2069-2079"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-JUN-2013) Terrestrial Biofilms Group, Friedrich Schiller
University of Jena, Neugasse 23, Jena, Thuringia 07743, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8381
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 106..210
/label=AmpR promoter
CDS 211..1068
/label=AmpR
/note="beta-lactamase"
rep_origin 1242..1830
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2080..2859)
/gene="ant1"
/label=ant1
/note="Spectinomycin 9-adenylyltransferase from
Staphylococcus aureus (strain N315). Accession#: P0A0D1"
gene complement(3968..5005)
/gene="amyE"
/label=amyE
/note="3` end"
CDS complement(5370..6017)
/gene="cat"
/label=cat
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
regulatory 6330..6728
/gene="rrnB"
/regulatory_class="promoter"
gene 6330..6728
/gene="rrnB"
/label=rrnB
CDS 6735..7424
/label=mKate2
/note="monomeric far-red fluorescent protein (Shcherbo et
al., 2009)"
regulatory 7450..7538
/regulatory_class="terminator"
regulatory 7545..7631
/regulatory_class="terminator"
gene complement(7747..8267)
/gene="amyE"
/label=amyE
/note="5` end"
This page is informational only.