pmKATE2rrnB vector (V004708)

LOCUS       V004708                 8381 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004708
VERSION     V004708
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   van Gestel J, Weissing FJ, Kuipers OP, Kovacs AT.
  TITLE     Density of founder cells affects spatial pattern formation and
            cooperation in Bacillus subtilis biofilms
  JOURNAL   ISME J 8 (10), 2069-2079 (2014)
   PUBMED   24694715
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Kovacs AT.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2013) Terrestrial Biofilms Group, Friedrich
            Schiller University of Jena, Neugasse 23, Jena, Thuringia 07743,
            Germany
REFERENCE   3  (bases 1 to 8381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "ISME J";
            date: "2014"; volume: "8"; issue: "10"; pages: "2069-2079"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-JUN-2013) Terrestrial Biofilms Group, Friedrich Schiller
            University of Jena, Neugasse 23, Jena, Thuringia 07743, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label="AmpR promoter"
     CDS             211..1068
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2080..2859)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     gene            complement(3968..5005)
                     /gene="amyE"
                     /label="amyE"
                     /note="3` end"
     CDS             complement(5370..6017)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6330..6728
                     /gene="rrnB"
                     /regulatory_class="promoter"
     gene            6330..6728
                     /gene="rrnB"
                     /label="rrnB"
     CDS             6735..7424
                     /label="mKate2"
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     regulatory      7450..7538
                     /regulatory_class="terminator"
     regulatory      7545..7631
                     /regulatory_class="terminator"
     gene            complement(7747..8267)
                     /gene="amyE"
                     /label="amyE"
                     /note="5` end"