LOCUS       V004708                 8381 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004708
VERSION     V004708
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   van Gestel J, Weissing FJ, Kuipers OP, Kovacs AT.
  TITLE     Density of founder cells affects spatial pattern formation and
            cooperation in Bacillus subtilis biofilms
  JOURNAL   ISME J 8 (10), 2069-2079 (2014)
   PUBMED   24694715
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Kovacs AT.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2013) Terrestrial Biofilms Group, Friedrich
            Schiller University of Jena, Neugasse 23, Jena, Thuringia 07743,
            Germany
REFERENCE   3  (bases 1 to 8381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "ISME J";
            date: "2014"; volume: "8"; issue: "10"; pages: "2069-2079"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-JUN-2013) Terrestrial Biofilms Group, Friedrich Schiller
            University of Jena, Neugasse 23, Jena, Thuringia 07743, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label="AmpR promoter"
     CDS             211..1068
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2080..2859)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     gene            complement(3968..5005)
                     /gene="amyE"
                     /label="amyE"
                     /note="3` end"
     CDS             complement(5370..6017)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6330..6728
                     /gene="rrnB"
                     /regulatory_class="promoter"
     gene            6330..6728
                     /gene="rrnB"
                     /label="rrnB"
     CDS             6735..7424
                     /label="mKate2"
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     regulatory      7450..7538
                     /regulatory_class="terminator"
     regulatory      7545..7631
                     /regulatory_class="terminator"
     gene            complement(7747..8267)
                     /gene="amyE"
                     /label="amyE"
                     /note="5` end"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct
     1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg
     1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat
     1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc
     2041 agaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga
     2101 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc
     2161 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa
     2221 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt
     2281 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat
     2341 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag
     2401 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac
     2461 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct
     2521 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact
     2581 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga
     2641 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac
     2701 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa
     2761 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat
     2821 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gttgattatg
     2881 ttcatataaa gtttatattg atactcaatt tacttaccct agattggaca tatacttaaa
     2941 ttactgttca ataaagctga ccgttagcgt ttaagtacat cctttcacaa tttgtctaca
     3001 gattaataat tattctttat tatacagatc gatcctctag acctaggcct taagatctga
     3061 tcatatgcat ccgcgggccc gggttaacgc gtaatccatg gatcaagaga caggatgagg
     3121 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggtgc cctgaatgaa
     3181 ctgcagaaag agctggtagt tggcgcactg ttcgaagaac tgccgatgtc cagtaagatt
     3241 cttactatgc tggttgaacc ggatgctggt aaagctactt gggttgctgc ttctacttat
     3301 ggtaccgata caactactgg tgaggaagtt aaaggagctc ttaaagaaat ccacttcagt
     3361 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
     3421 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
     3481 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
     3541 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     3601 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgacta
     3661 agaaaatgcc gtcaaatccg ctcgccatga cttcactaac gatgcctttg aaaatcttca
     3721 agttcttttc tactaattca aggcgtgtct caccaggttt ttggtttgct ccggcgcaaa
     3781 tgcagacaat atcagcatcc ttgcagggta tgtttctctt tgatgtcttt ttgtttgtga
     3841 agtatttcac atttatattg tgcaacactt cacaaacttt tgcaagagaa aagttttgtc
     3901 tgatttatga acaaaaaaga aaccatcatt gatggtttct ttcggtaagt cccgtctagc
     3961 cttgccctca atggggaaga gaaccgctta agcccgagtc attatataaa ccatttagca
     4021 cgtaatcaaa gccaggctga ttctgaccgg gcacttgggc gctgccatta ttaaaaatca
     4081 cttttgcgtt ggttgtatcc gtgtccgcag gcagcgtcag cgtgtaaatt ccgtctgcat
     4141 ttttagtcat tggttttcca ggccaagatc cggtcaattc aattactcgg ctcccatcat
     4201 gtttatagat ataagcattt acctggctcc aatgattcgg attttgatag ccgatggttt
     4261 tggccgacgc tggatctctt ttaacaaaac tgtatttctc ggtcctcgtt acaccatcac
     4321 tgttcgttcc ttttaacatg atggtgtatg ttttgccaaa ttggatctcc ttttccgatt
     4381 gtgaattgat ctccatcctt aaacgcctgt cgtctggtcc attattgatt tgataaacgg
     4441 cttttgttgt attcgcatct gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg
     4501 ttacacctgt tttgtaattc tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat
     4561 aaagcacagc tacagacctg gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa
     4621 atgaacccgc tccagcttta ttgtcatacc tgccatcagg caattttgtt gccgtattga
     4681 tagagacaga ggatgaacct gcatttgcca gcacaacgcc atgtgagccg cgctgattca
     4741 taaatatctg gttgtttcca ttcgggttcg agagttcctc aggctgtcca gccatcacat
     4801 tgtgaaatct attgaccgca gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc
     4861 ctatttggct tttccccggg aacctcacac catttccgcc tccctcaggt ctggaaaaga
     4921 aaagaggcgt actgcctgaa cgagaagcta tcaccgccca gcctaaacgg atatcatcat
     4981 cgctcatcca tgtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     5041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     5101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     5161 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     5221 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggagctg
     5281 taatataaaa accttcttca actaacgggg caggttagtg acattagaaa accgactgta
     5341 aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat
     5401 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc
     5461 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata
     5521 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc
     5581 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc
     5641 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca
     5701 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa
     5761 agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa
     5821 gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat
     5881 ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa
     5941 gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca
     6001 attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg
     6061 cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa
     6121 cataaatata tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg
     6181 gtgctttagt tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa
     6241 ggatttgagc gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg
     6301 ccgatgataa gctgtcaaac atgagaattc acatccctac gccagcgtta actaacaggt
     6361 tcaaagggtc agaacgaaac cgctcactct cagctttaat gctccccttg tggtcatcag
     6421 tatttagttc gtttctacta tacaagaaaa cgaaaaaaac aacaagatca catgactgat
     6481 gtatatgttc ttttaagaaa cttatatgat acacacgctt tagaaatcat ggcgaggatt
     6541 atagtttatt tgttttatag atttttttta aaaaactatt gcaataaata aatacaggtg
     6601 ttatattatt aaacgtcgct gatgcacagc ggacacaaac tagatgcttc aaaacaactt
     6661 gaaaaaagtt gttgacaaaa aagaagctga atgttatatt agtaaagcta agaggaggaa
     6721 ctactatggc tagctcagaa cttatcaagg aaaatatgca catgaaattg tacatggaag
     6781 gaacagtaaa taatcaccac tttaaatgta cctcagaagg agaaggaaaa ccatatgaag
     6841 gtactcaaac catgcgtatt aaggccgttg aaggtggacc attgcctttt gcctttgata
     6901 ttcttgctac atcttttatg tacggatcaa agacttttat caatcatacc caaggtatcc
     6961 cagatttctt taaacagtca tttcctgaag gatttacatg ggaacgtgtc acaacttatg
     7021 aagatggtgg agtattgaca gcaactcaag atacatcttt gcaagatggt tgtcttatct
     7081 acaatgtaaa gatccgtgga gttaattttc caagtaatgg tcctgttatg cagaaaaaga
     7141 cccttggatg ggaagcatct accgaaacat tatatcctgc cgatggtgga ttggaaggtc
     7201 gtgctgatat ggcattgaaa cttgtcggtg gaggtcacct tatctgtaat ttgaagacca
     7261 cataccgttc taaaaagcca gctaagaatc ttaagatgcc tggtgtttac tacgtcgatc
     7321 gtcgtttaga acgtatcaaa gaagcagata aggaaactta tgttgaacag cacgaagtag
     7381 ccgtcgcacg ttattgtgat ttgcctagta aattgggaca ccgttaataa ggccggccaa
     7441 taaagatcct aggacgccgc caagccagct taaacccagc tcaatgagct gggttttttg
     7501 tttgttaaaa atgaagaaga aactgtgaag cgtatttaac tagtaaaatc taagacatat
     7561 catgatttat aaaaacaaaa aaaccttgca ggatatgcaa ggcttctgga tgacccgtac
     7621 gggattcgaa ctccggatgt gagcgccggt cgctaccatt accagttggt ctggtgtcaa
     7681 aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg
     7741 tactatcgat cagaccagtt tttaatttgt gtgtttccat gtgtccagtt tggaatactc
     7801 ttaacctcat tggaaatcgc ggcataatca ctggtggtat gattgatgac cgcgtcaaca
     7861 atgaccttta tgccatattc ttcagcggct gcacacattt ctttaaattc ttgttcagta
     7921 cctaagtaac ggttgccaat ttgatacgat gtcggctgat acagccagta ccagttcgac
     7981 atgcttttat ctccttgatt cccttccttt acttggttaa tcggagatgt ctgaatggct
     8041 gtatatcctg catcatgaat atccttcata ttgtgtttta acgtattgaa cgaccaattc
     8101 catgcatgaa gaatggttcc gcttttgatc gacggtgctg taagctcatt cgatttgttc
     8161 gccgtttcag cactcgcagc cgccggtcct gccagaacca aatgaaacag caataaaaat
     8221 ccagcgaata acggcagtaa agaggttttg aatcgttttg caaacattct tgacactcct
     8281 tatttgattt tttgaagact tacttcggag tcaaaaatcc ctcttacttc attcttccgc
     8341 ttcctccttt caaaccgatg tgaagactgg agaattttgt t
//
