Basic Vector Information
- Vector Name:
- pMK33-NTAP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8775 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Veraksa A, Bauer A, Artavanis-Tsakonas S.
- Promoter:
- MT
pMK33-NTAP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK33-NTAP vector Sequence
LOCUS 40924_30995 8775 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMK33-NTAP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8775)
AUTHORS Veraksa A, Bauer A, Artavanis-Tsakonas S.
TITLE Analyzing protein complexes in Drosophila with tandem affinity
purification-mass spectrometry
JOURNAL Dev. Dyn. 232 (3), 827-834 (2005)
PUBMED 15704125
REFERENCE 2 (bases 1 to 8775)
AUTHORS Veraksa A, Bauer A, Artavanis-Tsakonas S.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2004) MGH Cancer Center, Harvard Medical School,
Bldg 149, 13th Street, Charlestown, MA 02129, USA
REFERENCE 3 (bases 1 to 8775)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8775)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Dyn.";
date: "2005"; volume: "232"; issue: "3"; pages: "827-834"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-AUG-2004) MGH Cancer Center, Harvard Medical School, Bldg 149,
13th Street, Charlestown, MA 02129, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8775
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..8
/label=NotI site
/note="NotI site"
rep_origin complement(865..1453)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1627..2484)
/label=AmpR
/note="beta-lactamase"
promoter complement(2485..2589)
/label=AmpR promoter
promoter 4071..4350
/label=copia promoter
/note="strong promoter from the Drosophila transposable
element copia (Sinclair et al., 1986)"
CDS 4396..5418
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
intron 5784..5849
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5979..5999
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 6424..6558
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 6559..6985
/label=MT promoter
/note="Drosophila metallothionein promoter"
CDS 7031..7204
/label=ProtA
/note="IgG-binding unit of Staphylococcus aureus protein A"
CDS 7208..7378
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
EAKKLNDAQAPK"
CDS 7415..7435
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 7442..7519
/label=CBP
/note="calmodulin-binding peptide"
CDS 7520..7534
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
misc_feature 7535..7558
/note="polylinker; unique cloning sites for XhoI, BamHI,
EcoRV, SpeI"
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