LOCUS       40924_30995        8775 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMK33-NTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Analyzing protein complexes in Drosophila with tandem affinity 
            purification-mass spectrometry
  JOURNAL   Dev. Dyn. 232 (3), 827-834 (2005)
  PUBMED    15704125
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2004) MGH Cancer Center, Harvard Medical School, 
            Bldg 149, 13th Street, Charlestown, MA 02129, USA
REFERENCE   3  (bases 1 to 8775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Dyn.";
            date: "2005"; volume: "232"; issue: "3"; pages: "827-834"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2004) MGH Cancer Center, Harvard Medical School, Bldg 149, 
            13th Street, Charlestown, MA 02129, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..8
                     /label=NotI site
                     /note="NotI site"
     rep_origin      complement(865..1453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1627..2484)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2485..2589)
                     /label=AmpR promoter
     promoter        4071..4350
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable
                     element copia (Sinclair et al., 1986)"
     CDS             4396..5418
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6424..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6559..6985
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             7031..7204
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             7208..7378
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     CDS             7415..7435
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             7442..7519
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             7520..7534
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    7535..7558
                     /note="polylinker; unique cloning sites for XhoI, BamHI,
                     EcoRV, SpeI"
ORIGIN
        1 gcggccgccg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
       61 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
      121 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat
      181 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg
      241 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
      301 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
      361 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc
      421 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg
      481 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
      541 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
      601 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
      661 ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1501 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1561 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1621 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1681 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1741 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1801 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1861 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1921 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1981 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2041 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2101 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2161 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2221 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     2281 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2341 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2401 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2461 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2521 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2581 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2641 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt
     2701 tgtacgaatt ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag
     2761 caaggcaaac ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac
     2821 tgcgtgattg atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat
     2881 gtcaaatgcg gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta
     2941 attgctgtgg aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact
     3001 tatgtacgta gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat
     3061 cagtcaagat gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt
     3121 tcaacaacta cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta
     3181 gtcacgtatt atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc
     3241 tcaaacattt cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat
     3301 ataagcaaac cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat
     3361 tgaggcgatt attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa
     3421 tttggaaatg tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt
     3481 tttcttcaga gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt
     3541 attttggaag gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg
     3601 gcccgaagct atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt
     3661 acattttcct ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc
     3721 tcttgcgaca caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag
     3781 aacgaaccga ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt
     3841 caattaagcc gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct
     3901 gtcctccaac tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc
     3961 ggttaaatta aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc
     4021 atgcttctgg gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata
     4081 tactattcaa cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc
     4141 cacacctttt atgccataaa acatattgta agagaatacc actcttttta ttccttcttt
     4201 ccttcttgta cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac
     4261 ttaaaatata aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa
     4321 ggaaatataa attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc
     4381 cgggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4441 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4501 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4561 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4621 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     4681 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     4741 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4801 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4861 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4921 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     4981 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5041 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5101 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5161 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5221 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5281 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5341 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5401 cgtccgaggg caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc
     5461 ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg
     5521 gtggcacagt tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg
     5581 tcgcagtgat tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca
     5641 cgcactcggg tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt
     5701 gggtgcagat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     5761 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     5821 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     5881 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     5941 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     6001 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     6061 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     6121 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     6181 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     6241 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     6301 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     6361 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6421 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6541 tcttatcatg tctggatcaa ttcgttgcag gacaggatgt ggtgcccgat gtgactagct
     6601 ctttgctgca ggccgtccta tcctctggtt ccgataagag acccagaact ccggcccccc
     6661 accgcccacc gccaccccca tacatatgtg gtacgcaagt aagagtgcct gcgcatgccc
     6721 catgtgcccc accaagagct ttgcatccca tacaagtccc caaagtggag aaccgaacca
     6781 attcttcgcg ggcagaacaa aagcttctgc acacgtctcc actcgaattt ggagccggcc
     6841 ggcgtgtgca aaagaggtga atcgaacgaa agacccgtgt gtaaagccgc gtttccaaaa
     6901 tgtataaaac cgagagcatc tggccaatgt gcatcagttg tggtcagcag caaaatcaag
     6961 tgaatcatct cagtgcaact aaagggggga tctcgacacc atggcaggcc ttgcgcaaca
     7021 cgatgaagcc gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     7081 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     7141 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc
     7201 gaaagtagac aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt
     7261 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
     7321 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt
     7381 agacgcgaat tgtgatatac ctacaactgc ttctgaaaat ttatattttc aaggtgaact
     7441 aaagagaaga tggaaaaaga atttcatagc cgtctcagca gccaaccgct ttaagaaaat
     7501 ctcatcctcc ggggcacttg atgatgacga taagctcgag ggatccgata tcactagttc
     7561 tagtcgacca tgaagatcaa gatcattgcc ccgccagagc gcaagtactc tgtctggatc
     7621 ggtggctcca tcctggcttc gctgtccacc ttccagcaga tgtggatctc caagcaggag
     7681 tacgacgagt ccggcccctc cattgtgcac cgcaagtgct tctaagaagg atcgcttgtc
     7741 tgggcaagag gatcaggatc gggatggtct tgattctgct ggaggaggag gaggagaagt
     7801 cgaggaagca gcagcgaaag tgcaagtgcg agtggtggaa gtttggagtg cagcacaaca
     7861 aaatcaacaa caacaccaac tacaagatga aaagagcgga accacctgca caccatcatc
     7921 actatcatca tcgttttggg cgcatgttgt gtggttccag cgtattaata taattaattt
     7981 attccacatg agatatgata tgatatacta tgtatttttt gttttttttt tatttgtaaa
     8041 cctttaatat aacaagaact acaaaaaatg aaaatgagcg aaaatgcata ttctgccatt
     8101 ccacacacac accaacaaca cccaacacac gcacacccac aagcttacac acacacattc
     8161 gcggcatgac aaggacatca agataaagaa gaacttaaag aagatatttc ccaaagcgca
     8221 aaaagaacac acacacattg caaaacacaa acaacacact agcgttttgt acaattcgtc
     8281 agcaacctta tgtattattt tttaattatg atgtaattat aaacaaagtg aaacaaaaat
     8341 atgaaaacaa aaaggaaaat caaatctgtc ttctctttct cccgctctcc tcgctctctg
     8401 ctgctaacct cgccctctcc tctctcatct ttttgtctgt ctctcttcca catttttgcc
     8461 ggccggcaaa ataataaccc acacacactc acacttggct gcagtttcgc gtgcgatatt
     8521 cacacacatt caagcataca agcatacata catatgtatt ttttttttat ttgtacactt
     8581 ttctaattgc atgcgtatcg attgataagt ttacgcctga aaatgttaat taaaatgtga
     8641 aaatgcaact gaaaaactga tgaaatgaaa caacaacaag cgaacaattt gctacatgtg
     8701 tattgtctaa caaccgttac tgcaacggtt gcttcggaaa agggtgaaga ggaagagggc
     8761 acgaggtcga ctaga
//
