Basic Vector Information
- Vector Name:
- pMK-RQ_MXAN_1530-1529exp
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4604 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lorenzen W.
pMK-RQ_MXAN_1530-1529exp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK-RQ_MXAN_1530-1529exp vector Sequence
LOCUS 40924_30945 4604 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pMK-RQ_MXAN_1530-1529exp, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4604) AUTHORS Lorenzen W. TITLE A multifunctional enzyme is involved in bacterial ether lipid biosynthesis JOURNAL Unpublished REFERENCE 2 (bases 1 to 4604) AUTHORS Lorenzen W. TITLE Direct Submission JOURNAL Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur Molekulare Biowissenschaften, Goethe Universtaet Frankfurt, Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany REFERENCE 3 (bases 1 to 4604) TITLE Direct Submission REFERENCE 4 (bases 1 to 4604) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur Molekulare Biowissenschaften, Goethe Universtaet Frankfurt, Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: ??? v. ??? Sequencing Technology :: unknown ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4604 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" /translation="IEGR" CDS 381..1046 /codon_start=1 /product="hypothetical protein" /label=hypothetical protein /note="MXAN_1530" /protein_id="AHY21927.1" /translation="MSSKAAFFDVDGTLVKTNVVHVYAYYAMNRGSVLGIAGRTLSTAL SVPLFGVMDAVDRKTFNEFFYRYYAGLSEDRLVTIAEDMFEDVLQPALFEQTQDLIDQA RRSGCKIVLVTGALDFTMRPLARHLGADDMIANKMQFVGGKATGKVIPPIIEGANKANA IRAYCTKEGLSLDKCHGYSDSASDYAMLAVVGRPTAVNPDLRLRSIARAYNWPILDLK" CDS 1071..2681 /codon_start=1 /product="hypothetical protein" /label=hypothetical protein /note="MXAN_1529" /protein_id="AHY21925.1" /translation="MRPFKTLQKLYDEESQVVITEKGSPPRVLFHGENFLQEDLPVGTR VIFPRPPLAGVPNVKAAIRYAINNPEGMEPLHALLKPGMRLTCVIDDISVPLPPMVTPD VRQTILEIVLELAADSGVDDVHLIIANALHRRMTEGEMKRMVGEKIFDAYYPDRYYNHD AEDPDGMVALERTSHGEEVSVNRRVAESDLIVYVNVNFVPMNGGHKSMGTGVSNYASLR HHHNPKTIRASDSYMEPKTSALYKSNERIGRNIDKHLKVFHIETALNNRMFGGPTDFLA KKEEDYTEADRLKFQAMRFALSKLPRAAARKVLNSVPAPYDVTGVYAGATEPTHAKTLE TSYKQYVVPVEGQSDIVIFPIPFISPYSVNSILNPLLVQVMGLGYFYNLNRGVPLVKKG GVLILLHPAYDEFDPEHHPSYIEFFHRLLPETRDSMKLEHKYEREFAENPSYVHLYRKG NAYHGVHPFYMWYWGENGRQHVGKVIVAGAENNHVPALMGWDRTDTLTEAIEEARGFMG RSATISLLRIAPTVMVDVK" rep_origin complement(2920..3508) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3685..4476) /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(4477..4581) /label=AmpR promoter
This page is informational only.