LOCUS       40924_30945        4604 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMK-RQ_MXAN_1530-1529exp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Lorenzen W.
  TITLE     A multifunctional enzyme is involved in bacterial ether lipid 
            biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Lorenzen W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur 
            Molekulare Biowissenschaften, Goethe Universtaet Frankfurt, 
            Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany
REFERENCE   3  (bases 1 to 4604)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4604)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2014) Molekulare Biotechnologie / Institut fur Molekulare 
            Biowissenschaften, Goethe Universtaet Frankfurt, Max-von-Laue 
            Strasse 9, Frankfurt am Main, Hessen 60438, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ??? v. ???
            Sequencing Technology :: unknown
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             381..1046
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1530"
                     /protein_id="AHY21927.1"
                     /translation="MSSKAAFFDVDGTLVKTNVVHVYAYYAMNRGSVLGIAGRTLSTAL
                     SVPLFGVMDAVDRKTFNEFFYRYYAGLSEDRLVTIAEDMFEDVLQPALFEQTQDLIDQA
                     RRSGCKIVLVTGALDFTMRPLARHLGADDMIANKMQFVGGKATGKVIPPIIEGANKANA
                     IRAYCTKEGLSLDKCHGYSDSASDYAMLAVVGRPTAVNPDLRLRSIARAYNWPILDLK"
     CDS             1071..2681
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1529"
                     /protein_id="AHY21925.1"
                     /translation="MRPFKTLQKLYDEESQVVITEKGSPPRVLFHGENFLQEDLPVGTR
                     VIFPRPPLAGVPNVKAAIRYAINNPEGMEPLHALLKPGMRLTCVIDDISVPLPPMVTPD
                     VRQTILEIVLELAADSGVDDVHLIIANALHRRMTEGEMKRMVGEKIFDAYYPDRYYNHD
                     AEDPDGMVALERTSHGEEVSVNRRVAESDLIVYVNVNFVPMNGGHKSMGTGVSNYASLR
                     HHHNPKTIRASDSYMEPKTSALYKSNERIGRNIDKHLKVFHIETALNNRMFGGPTDFLA
                     KKEEDYTEADRLKFQAMRFALSKLPRAAARKVLNSVPAPYDVTGVYAGATEPTHAKTLE
                     TSYKQYVVPVEGQSDIVIFPIPFISPYSVNSILNPLLVQVMGLGYFYNLNRGVPLVKKG
                     GVLILLHPAYDEFDPEHHPSYIEFFHRLLPETRDSMKLEHKYEREFAENPSYVHLYRKG
                     NAYHGVHPFYMWYWGENGRQHVGKVIVAGAENNHVPALMGWDRTDTLTEAIEEARGFMG
                     RSATISLLRIAPTVMVDVK"
     rep_origin      complement(2920..3508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3685..4476)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4477..4581)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg aattctttaa atgagcagca aagcagcctt ctttgatgtt gatggcaccc
      421 tggttaaaac caatgttgtt catgtgtatg cctactatgc aatgaatcgt ggtagcgttc
      481 tgggtattgc aggtcgtacc ctgagcaccg cactgagcgt tccgctgttt ggtgttatgg
      541 atgcagttga tcgtaaaacc ttcaacgagt tcttctatcg ttattatgca ggtctgagcg
      601 aagatcgtct ggttaccatt gccgaagata tgtttgaaga tgttctgcag cctgcactgt
      661 ttgaacagac ccaggatctg attgatcagg cacgtcgtag cggttgtaaa attgttctgg
      721 tgaccggtgc actggatttc accatgcgtc cgctggcacg tcatctgggt gcagatgata
      781 tgattgccaa taaaatgcag tttgtgggtg gtaaagcaac cggtaaagtt attccgccta
      841 ttattgaagg tgccaacaaa gcaaatgcaa ttcgtgcata ttgtacgaaa gaaggtctga
      901 gtctggataa atgtcatggt tatagcgata gcgcaagcga ttatgcaatg ctggcagttg
      961 ttggtcgtcc gaccgcagtt aatccggatc tgcgtctgcg tagcattgca cgtgcctata
     1021 attggccgat tctggatctg aaatgaggag gccaattgct tcgcctttaa atgcgtccgt
     1081 tcaaaaccct gcagaaactg tatgatgaag aaagccaggt tgtgattacc gaaaaaggta
     1141 gccctccgcg tgttctgttt catggcgaaa actttctgca agaagatctg ccggttggca
     1201 cccgtgttat ttttccgcgt ccgcctctgg caggcgttcc gaatgttaaa gcagcaattc
     1261 gttatgccat taataacccg gaaggtatgg aaccgctgca tgcactgctg aaaccgggta
     1321 tgcgtctgac ctgtgttatt gatgatattt cagttccgct gcctccgatg gttacaccgg
     1381 atgttcgtca gaccattctg gaaattgtgc tggaactggc agcagatagc ggtgttgatg
     1441 atgttcatct gattattgca aatgcactgc atcgtcgtat gaccgaaggt gaaatgaaac
     1501 gtatggttgg cgaaaaaatc ttcgatgcct attatccgga tcgctattac aatcatgatg
     1561 cggaagatcc tgatggtatg gttgcactgg aacgtaccag ccatggtgaa gaagttagcg
     1621 ttaatcgtcg tgttgccgaa agcgatctga tcgtttatgt gaatgttaac tttgtgccga
     1681 tgaacggtgg tcataaaagc atgggcaccg gtgttagcaa ttatgcaagc ctgcgtcatc
     1741 atcataatcc gaaaaccatt cgtgcaagcg atagctatat ggaacctaaa accagcgcac
     1801 tgtataaaag caatgaacgt attggtcgca acatcgacaa acacctgaaa gtgtttcata
     1861 ttgaaacggc actgaacaat cgtatgtttg gcggtccgac cgattttctg gcaaaaaaag
     1921 aagaagatta taccgaagcc gatcgcctga aattccaggc aatgcgtttt gcactgagca
     1981 aactgcctcg tgcagcagca cgtaaagttc tgaatagcgt tccggcaccg tatgatgtta
     2041 ccggtgttta tgccggtgca accgaaccga cccatgcaaa aaccctggaa accagctata
     2101 aacagtatgt tgttccggtt gaaggtcaga gcgatattgt gatttttccg attccgttta
     2161 ttagcccgta tagcgtgaat agcattctga atccgctgct ggttcaggtt atgggtctgg
     2221 gttatttcta taatctgaat cgcggtgttc cgctggtgaa aaaaggtggt gttctgattc
     2281 tgctgcatcc ggcatatgat gaatttgatc cggaacatca cccgagctat atcgaatttt
     2341 ttcatcgtct gctgccggaa acccgtgata gcatgaaact ggaacataaa tatgaacgcg
     2401 aatttgcgga aaatccgagc tatgtgcatc tgtatcgtaa aggtaatgcc tatcatggtg
     2461 tgcacccgtt ttatatgtgg tattggggtg aaaatggtcg tcagcatgtt ggtaaagtga
     2521 ttgttgcggg tgcagaaaat aatcatgttc ctgcactgat gggttgggat cgtaccgata
     2581 ccctgaccga agccattgaa gaggcacgtg gttttatggg tcgtagcgca accattagcc
     2641 tgctgcgtat tgcaccgacc gttatggttg atgttaaata acaattgcct gcaggctggg
     2701 cctcatgggc cttcctttca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2761 attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg
     2821 actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca
     2881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3661 gtaaacttgg tctgacagtt attagaaaaa ttcatccagc agacgataaa acgcaatacg
     3721 ctggctatcc ggtgccgcaa tgccatacag caccagaaaa cgatccgccc attcgccgcc
     3781 cagttcttcc gcaatatcac gggtggccag cgcaatatcc tgataacgat ccgccacgcc
     3841 cagacggccg caatcaataa agccgctaaa acggccattt tccaccataa tgttcggcag
     3901 gcacgcatca ccatgggtca ccaccagatc ttcgccatcc ggcatgctcg ctttcagacg
     3961 cgcaaacagc tctgccggtg ccaggccctg atgttcttca tccagatcat cctgatccac
     4021 caggcccgct tccatacggg tacgcgcacg ttcaatacga tgtttcgcct gatgatcaaa
     4081 cggacaggtc gccgggtcca gggtatgcag acgacgcatg gcatccgcca taatgctcac
     4141 tttttctgcc ggcgccagat ggctagacag cagatcctga cccggcactt cgcccagcag
     4201 cagccaatca cggcccgctt cggtcaccac atccagcacc gccgcacacg gaacaccggt
     4261 ggtggccagc cagctcagac gcgccgcttc atcctgcagc tcgttcagcg caccgctcag
     4321 atcggttttc acaaacagca ccggacgacc ctgcgcgctc agacgaaaca ccgccgcatc
     4381 agagcagcca atggtctgct gcgcccaatc atagccaaac agacgttcca cccacgctgc
     4441 cgggctaccc gcatgcaggc catcctgttc aatcatactc ttcctttttc aatattattg
     4501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4561 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
