Basic Vector Information
- Vector Name:
- pMK-RQ_MXAN_1527_1531exp
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4318 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lorenzen W.
pMK-RQ_MXAN_1527_1531exp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK-RQ_MXAN_1527_1531exp vector Sequence
LOCUS 40924_30940 4318 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMK-RQ_MXAN_1527_1531exp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4318)
AUTHORS Lorenzen W.
TITLE A multifunctional enzyme is involved in bacterial ether lipid
biosynthesis
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4318)
AUTHORS Lorenzen W.
TITLE Direct Submission
JOURNAL Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur
Molekulare Biowissenschaften, Goethe Universtaet Frankfurt,
Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany
REFERENCE 3 (bases 1 to 4318)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4318)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(28-MAR-2014) Molekulare Biotechnologie / Institut fur Molekulare
Biowissenschaften, Goethe Universtaet Frankfurt, Max-von-Laue
Strasse 9, Frankfurt am Main, Hessen 60438, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: ??? v. ???
Sequencing Technology :: unknown
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4318
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 343..354
/codon_start=1
/label=Factor Xa site
/note="Factor Xa recognition and cleavage site"
/translation="IEGR"
CDS 373..1401
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="MXAN_1527"
/protein_id="AHY21922.1"
/translation="MKLLVTGGTGFLGTHLVPRLVAAGHEVRLIGRSRPSGAPYAGTEY
VPGDLKNRDAVRRALEGVDAVYHLAGLVSFQPKDARKMFELHVDSTRELLRDVREAGVK
RVVLASTSGTIAVSKEAHVRDESADYPITVVGQWPYYLSKIYEEKLALAYCRKHEIPLV
VLNPSLLMGPGDDRLSSTWTVVKFLNREIPAMPGGGISFVDARDAADAFVQALTRGEVY
GRHLMGVNLSMKDFFQRLERLSGVPAPKLKLPSKVNVLGGKLLERWAKVRGTTPTLDPQ
EIDIGEHWFWLDASKAEAELGFRARDIQETLSETVQHIYGKMPPQSLPGTKGRLAELRE
NT"
CDS 1417..2397
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="MXAN_1531"
/protein_id="AHY21923.1"
/translation="MRALITGAGGFLGMWLAKALAARGDSVTCLLRPGGDASGLAGLTY
TRVEGDVTVPSTLSAAVAGQDVVFHLAGARRGATRDDFMRVNAEGTRHLCEAMVAAGHR
PRLVLTGSLAACGPSTPTRPHVEEDAFHPHEWYGESKAEAERIAFSYGDRLPVTVSRPP
RILGPGDRENLPFFKMAQRGIRLELSGGPRPLTMVDVEDVVDILLLQATHPAAIGEAFF
CAGPGEPLSLEKVQDLGAQALGLTPRTVRVSPALLRAMATAADGVSQLTGRKLALSRKM
AKQLLAPAWTCSGAKAERLLGFHPQRDLADSIRRSVQWYREQGWL"
rep_origin complement(2634..3222)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3399..4190)
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(4191..4295)
/label=AmpR promoter
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