LOCUS       40924_30940        4318 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMK-RQ_MXAN_1527_1531exp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Lorenzen W.
  TITLE     A multifunctional enzyme is involved in bacterial ether lipid 
            biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Lorenzen W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2014) Molekulare Biotechnologie / Institut fur 
            Molekulare Biowissenschaften, Goethe Universtaet Frankfurt, 
            Max-von-Laue Strasse 9, Frankfurt am Main, Hessen 60438, Germany
REFERENCE   3  (bases 1 to 4318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2014) Molekulare Biotechnologie / Institut fur Molekulare 
            Biowissenschaften, Goethe Universtaet Frankfurt, Max-von-Laue 
            Strasse 9, Frankfurt am Main, Hessen 60438, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ??? v. ???
            Sequencing Technology :: unknown
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             373..1401
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1527"
                     /protein_id="AHY21922.1"
                     /translation="MKLLVTGGTGFLGTHLVPRLVAAGHEVRLIGRSRPSGAPYAGTEY
                     VPGDLKNRDAVRRALEGVDAVYHLAGLVSFQPKDARKMFELHVDSTRELLRDVREAGVK
                     RVVLASTSGTIAVSKEAHVRDESADYPITVVGQWPYYLSKIYEEKLALAYCRKHEIPLV
                     VLNPSLLMGPGDDRLSSTWTVVKFLNREIPAMPGGGISFVDARDAADAFVQALTRGEVY
                     GRHLMGVNLSMKDFFQRLERLSGVPAPKLKLPSKVNVLGGKLLERWAKVRGTTPTLDPQ
                     EIDIGEHWFWLDASKAEAELGFRARDIQETLSETVQHIYGKMPPQSLPGTKGRLAELRE
                     NT"
     CDS             1417..2397
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="MXAN_1531"
                     /protein_id="AHY21923.1"
                     /translation="MRALITGAGGFLGMWLAKALAARGDSVTCLLRPGGDASGLAGLTY
                     TRVEGDVTVPSTLSAAVAGQDVVFHLAGARRGATRDDFMRVNAEGTRHLCEAMVAAGHR
                     PRLVLTGSLAACGPSTPTRPHVEEDAFHPHEWYGESKAEAERIAFSYGDRLPVTVSRPP
                     RILGPGDRENLPFFKMAQRGIRLELSGGPRPLTMVDVEDVVDILLLQATHPAAIGEAFF
                     CAGPGEPLSLEKVQDLGAQALGLTPRTVRVSPALLRAMATAADGVSQLTGRKLALSRKM
                     AKQLLAPAWTCSGAKAERLLGFHPQRDLADSIRRSVQWYREQGWL"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3399..4190)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4191..4295)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc atatgaaact gctggttacc ggtggtacag gttttctggg cacccatctg
      421 gttccgcgtc tggttgcagc aggtcatgaa gttcgtctga ttggtcgtag ccgtccgagt
      481 ggtgcaccgt atgcaggcac cgaatatgtt ccgggtgatc tgaaaaatcg tgatgcagtt
      541 cgtcgtgcac tggaaggtgt tgatgccgtt tatcatctgg caggtctggt tagctttcag
      601 ccgaaagatg cacgtaaaat gtttgaactg catgttgata gcacccgtga actgctgcgt
      661 gatgttcgtg aagccggtgt taaacgtgtt gttctggcaa gcaccagcgg caccattgca
      721 gttagcaaag aagcacacgt tcgcgacgaa agcgcagatt atccgattac cgttgttggt
      781 cagtggccgt attacctgag caaaatctat gaagaaaaac tggcactggc ctattgccgc
      841 aaacatgaaa ttccgctggt tgttctgaat ccgagcctgc tgatgggtcc tggtgatgat
      901 cgtctgagca gcacctggac cgttgttaaa tttctgaatc gcgaaattcc ggcaatgcct
      961 ggtggtggta ttagctttgt tgatgcacgt gatgccgcag atgcatttgt tcaggcactg
     1021 acccgtggtg aagtttatgg tcgtcatctg atgggtgtta atctgagcat gaaagatttt
     1081 tttcagcgtc tggaacgtct gagcggtgtt ccggcaccga aactgaaact gccgagcaaa
     1141 gttaatgttc tgggtggtaa actgctggaa cgttgggcaa aagttcgtgg caccaccccg
     1201 accctggatc cgcaagaaat tgatattggt gaacattggt tttggctgga tgcaagcaaa
     1261 gcagaagccg aactgggttt tcgtgcccgt gatattcaag aaaccctgag cgaaaccgtt
     1321 cagcatattt atggtaaaat gcctccgcag agcctgcctg gcaccaaagg tcgtctggca
     1381 gaactgcgtg aaaataccta aaggaggatt aagcttatgc gtgcactgat taccggtgcc
     1441 ggtggctttc tgggtatgtg gctggcaaaa gcactggcag cacgtggtga tagcgttacc
     1501 tgtctgctgc gtccgggtgg tgatgcaagc ggtctggcag gcctgaccta tacccgtgtt
     1561 gaaggtgatg ttaccgttcc gagcaccctg agtgcagcag ttgcaggtca ggatgttgtg
     1621 tttcacctgg caggcgcacg tcgtggtgca acccgtgatg attttatgcg tgttaatgcc
     1681 gaaggcaccc gtcatctgtg tgaagcaatg gtggcagccg gtcatcgtcc tcgtctggtt
     1741 ctgaccggta gcctggcagc ctgtggtccg agcacaccga cccgtccgca tgttgaagaa
     1801 gatgcctttc atccgcatga atggtatggt gaaagtaaag ccgaagcaga acgtattgca
     1861 tttagctatg gtgatcgcct gccggttacc gttagccgtc ctccgcgtat tctgggtccg
     1921 ggtgaccgcg aaaatctgcc gtttttcaaa atggcacagc gtggtattcg cctggaactg
     1981 agtggtggtc cgcgtccgct gaccatggtt gatgtggaag atgttgttga tattctgctg
     2041 ctgcaagcaa cccatccggc agcaattggt gaagcctttt tttgtgcagg accgggtgaa
     2101 ccgctgagcc tggaaaaagt tcaggatctg ggtgcacagg cactgggtct gacaccgcgt
     2161 accgttcgtg ttagtcctgc cctgctgcgt gcaatggcaa ccgcagcaga tggtgttagc
     2221 cagctgaccg gtcgtaaact ggccctgagc cgtaaaatgg caaaacagct gctggcaccg
     2281 gcatggacct gtagcggtgc aaaagccgaa cgtctgctgg gtttccatcc gcagcgtgat
     2341 ctggcagata gcattcgtcg tagcgttcag tggtatcgtg aacagggttg gctgtaaaag
     2401 cttctcgagc tgggcctcat gggccttcct ttcactgccc gctttccagt cgggaaacct
     2461 gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg cgctctccgc
     2521 ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg tgcctaatga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3361 taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc cagcagacga
     3421 taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag aaaacgatcc
     3481 gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat atcctgataa
     3541 cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc attttccacc
     3601 ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc atccggcatg
     3661 ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc ttcatccaga
     3721 tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat acgatgtttc
     3781 gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg catggcatcc
     3841 gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc ctgacccggc
     3901 acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag caccgccgca
     3961 cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg cagctcgttc
     4021 agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc gctcagacga
     4081 aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc aaacagacgt
     4141 tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat actcttcctt
     4201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
