Basic Vector Information
- Vector Name:
- pMH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3565 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Gortchakov AA, Kaltenhauser J, Eggert H, Saumweber H.
pMH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMH vector Sequence
LOCUS 40924_30800 3565 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pMH, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3565)
AUTHORS Gortchakov AA, Kaltenhauser J, Eggert H, Saumweber H.
TITLE pRich, a novel convenient Escherichia coli protein expression vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3565)
AUTHORS Gortchakov AA, Kaltenhauser J, Eggert H, Saumweber H.
TITLE Direct Submission
JOURNAL Submitted (27-MAR-2003) Lab. Molecular Cytogenetics, The Institute
of Cytology and Genetics, SD RAS, Lavrentjeva, 10, Novosibirsk
630090, Russia
REFERENCE 3 (bases 1 to 3565)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3565)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-MAR-2003) Lab. Molecular Cytogenetics, The Institute of Cytology
and Genetics, SD RAS, Lavrentjeva, 10, Novosibirsk 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3565
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 26..44
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 76..98
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 118..147
/codon_start=1
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
CDS 154..183
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 190..219
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 223..240
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
misc_feature 242..257
/label=polylinker
/note="polylinker"
terminator 313..407
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS 451..1107
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1175..1261
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1416..1556
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1742..2330)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2504..3361)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3362..3466)
/label=AmpR promoter
This page is informational only.