LOCUS       40924_30800        3565 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Gortchakov AA, Kaltenhauser J, Eggert H, Saumweber H.
  TITLE     pRich, a novel convenient Escherichia coli protein expression vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Gortchakov AA, Kaltenhauser J, Eggert H, Saumweber H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2003) Lab. Molecular Cytogenetics, The Institute 
            of Cytology and Genetics, SD RAS, Lavrentjeva, 10, Novosibirsk 
            630090, Russia
REFERENCE   3  (bases 1 to 3565)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2003) Lab. Molecular Cytogenetics, The Institute of Cytology
            and Genetics, SD RAS, Lavrentjeva, 10, Novosibirsk 630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             76..98
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             118..147
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             154..183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             190..219
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             223..240
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    242..257
                     /label=polylinker
                     /note="polylinker"
     terminator      313..407
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             451..1107
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1175..1261
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1416..1556
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1742..2330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2504..3361)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3362..3466)
                     /label=AmpR promoter
ORIGIN
        1 cctcgagatc tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt
       61 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggg cggacacgaa
      121 caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac
      181 ttgaacggtg aacaaaaatt aatctcagaa gaagacttga accaccatca ccaccaccat
      241 ggcggccgcg gatccgcatg cgagctcggt accccgggtc gacctgcagc caagcttaat
      301 tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      361 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagcttg
      421 gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      481 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      541 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      601 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      661 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      721 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      781 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      841 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      901 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      961 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     1021 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     1081 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
     1141 acgcctgggg taatgactct ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa
     1201 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1261 tccgccctct agagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1321 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1381 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1441 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     1501 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     1561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2161 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3481 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3541 gtatcacgag gccctttcgt cttca
//
